Corpora amylacea(CA) in the human brain are granular bodies formed by polyglucosan aggregates that amass waste products of different origins. They are generated by astrocytes, mainly during aging and neurodegenerative conditions, and are located predominantly in periventricular and subpial regions. This study shows that CA are released from these regions to the cerebrospinal fluid and are present in the cervical lymph nodes, into which cerebrospinal fluid drains through the meningeal lymphatic system. We also show that CA can be phagocytosed by macrophages. We conclude that CA can act as containers that remove waste products from the brain and may be involved in a mechanism that cleans the brain. Moreover, we postulate that CA may contribute in some autoimmune brain diseases, exporting brain substances that interact with the immune system, and hypothesize that CA may contain brain markers that may aid in the diagnosis of certain brain diseases.

Fruit of varieties Golden Delicious and Granny Smith, were harvested at commercial maturity in two comecutive years (1993 and 1994). The aroma components obtained were analyzed by the dynamic headspace method and quantified by gas chromatograph-mass spectrometry. In Golden Delicious the total esters represented more than 80% of the total volatile components in both years, consistent with Drawen's classification (1975). However, Granny Smith did not, since the esters made up 72 and 88% of total volatiles in the years 1993 and 1994, respectively. The aroma components are different in class and quuntity and give a characteristic sensorial perception for each type offruit and variety. In Golden Delicious, ethyl propionate and butyl acetate gave a fnrity aroma in 1993 and ethyl acetate, ethyl propionate and propyl acetate were the predominant compounds in 1994. In Granny Smith the aroma composition was slighfly different in the two years. In 1993 the aromatic profile did not show the predominance of any compound, while in 1994 it was more fruity due to the higher content of ethyl propionare and propyl acetate.

No abstract

Starking Delicious apples were cold-stored in semicommercial chambers in normal atmosphere and in different controlled atmospheres in which oxygen and carbon dioxide were held at 1%, 2%, and 3% for 3, 5, and 7 months. After storage, fruit was kept at ambient temperature (20 degrees C) for 1, 5, and 10 days. The volatile emission was collected and analyzed. After all storage periods and conditions, the total aromatic volatile emission was higher than at commercial harvest. The highest emission of volatile components was obtained after 5 months of storage, in which controlled-atmosphere conditions gave a lower concentration than normal cold storage. Esters were the main volatiles detected (>98%). Acetate ester concentrations after all storage periods assayed were higher at 3% and 2% O(2)/CO(2). The components that contributed most to the characteristic aroma of Starking Delicious were ethyl 2-methylbutyrate, ethyl butyrate, and ethyl hexanoate. Their concentrations were higher after 5 months of storage and also during poststorage at ambient temperature (20 degrees C).

The antagonistic effect on the pheromone response and catabolism of male European corn borers, Ostrinia nubilalis, by several trifluoromethyl ketones is reported. (Z)-11-Tetradecenyl trifluoromethyl ketone (Z11-14:TFMK), the most closely related analogue of the main component of the pheromone, elicits a remarkable disruptive effect on close approach and source contact of males flying to a source baited with mixtures of the pheromone and the antagonist in 5:1 and 10:1 ratios. In this experiment, the male displayed an erratic flight track with frequent counter turns and intersections with the plume. In the field, the TFMK significantly lowered the number of males caught when mixed with the pheromone in a 10:1 ratio in comparison with the natural attractant. The compound was also a good inhibitor of the antennal esterase of the insect with a IC(50) value of 0.28 muM. The homologous (Z)-10-tridecenyl trifluoromethyl ketone, with one carbon less in the chain, also elicited an antagonistic effect in the wind tunnel, but in the field, the results were not conclusive. The effect induced was lower than the one displayed by Z11-14:TFMK including the activity as the esterase inhibitor (IC(50) value of 7.55 muM). The saturated tetradecyl trifluoromethyl ketone, tetradecyltrifluoropyruvamide, and (Z)-11-2-thiatetradecenyl trifluoromethyl ketone resulted completely inactive. The results obtained in conjunction to the previously shown low toxicity to mice by related trifluoromethyl ketones provide new important data for the putative utilization of these chemicals as new pest control agents.

No abstract

A new pheromone antagonist of the codling moth Cydia pomonella is reported. Presaturation of the antennae of the insects with vapors of the antagonist (E,E)-8,10-dodecadienyl trifluoromethyl ketone, analogue of the main component of the pheromone (codlemone), resulted in lower electrophysiological responses to the pheromone relative to untreated insects. In the wind tunnel, the compound elicited a remarkable disruptive effect on all types of behavior of males flying toward a source baited with a pheromone/antagonist blend in 1:1, 1:5, and 1:10 ratios. The insects displayed erratic flights in the presence of the antagonist, as shown by their flight parameters in comparison to insects attracted to the pheromone alone. In the field, traps baited with mixtures of 1:10 codlemone/antagonist attracted considerably lower numbers of males than the natural attractant. The antagonist, however, did not inhibit the pheromone-degrading enzymes present in male antennae, suggesting that trifluoromethyl ketones are not sufficiently electrophilic to produce a stable intermediate adduct with a cysteine residue of the enzyme, a mechanism previously proposed for oxidase inhibition in insects. Overall and taking into account our previous reports and, particularly, the reduction in damage induced in maize fields by a trifluoromethyl ketone analogue of the pheromone of Sesamia nonagrioides (Sole, J.; Sans, A.; Riba, M.; Rosa, E.; Bosch, M. P.; Barrot, M.; Palencia, J.; Castella, J.; Guerrero, A. Reduction of damage by the Mediterranean corn borer, Sesamia nonagrioides , and the European corn borer, Ostrinia nubilalis , in maize fields by a trifluoromethyl ketone pheromone analog . Entomol. Exp. Appl. 2008, 126, 28-39), the antagonist might be a new candidate to consider in future strategies to control the codling moth.

The electrophysiological and behavioural responses of male Sesamia nonagrioides Lefèbvre (Lepidoptera: Noctuidae) to several synthetic blends of its sex pheromone components were investigated. Electroantennogram (EAG) recordings showed the greatest response to a 77:8:10:5 blend of the four compounds found in the female glands: (Z)‐11‐hexadecen‐1‐yl acetate, (Z)‐11‐hexadecen‐1‐ol, (Z)‐11‐hexadecenal and dodecyl acetate. A logistic equation was fitted to the dose‐response data and differences in relative EAG responses were analysed by deriving five parameters of this equation. Flight responses of males were also evaluated in a wind tunnel where the proposed blend (77:8:10:5) also elicited the best response. Field tests confirmed the laboratory findings as the 77:8:10:5 blend captured more S. nonagrioides males than other pheromone mixtures and showed more selectivity for male S. nonagrioides over other noctuid species, Discestra trifolii Rottenberg and Lacanobia oleracea L., but not for Mythimna unipuncta Haworth, a species with a sex pheromone composition almost identical to that of S. nonagrioides.