ObjectiveEuploid embryo transfers yield better implantation rates. In Brazil,
morphological evaluation is performed to select the best embryos, since
genetic analysis is still an expensive procedure. This study aimed to
evaluate whether there is an association between trophectoderm morphology
and ploidy status.MethodsThe study included 113 blastocysts formed in D5/D6 from 58 in
vitro fertilization cycles held from January/2016 to May/2017.
All patients with indication for PGD/PGS were included in the study. The
mean age of the female patients was 37.04±5.65years. Biopsied
blastocysts were categorized for morphology. Cells were sent for genetic
analysis using the CGH array, SNP array or NGS techniques. Statistical
analysis was performed using the chi square test, and statistical
significance was assigned to differences with
p≤0.05.ResultsChromosome analysis revealed that 44 (38.9%) blastocysts were euploid.
Blastocysts with trophectoderm grades A, B, and C had euploidy rates of
71.43%, 60% and 19.67%, respectively (p≤0.05).ConclusionAlthough the best trophectoderm morphology grades had higher euploidy rates,
this indicator alone is not enough to warrant embryo genetic viability.
ObjectiveProgesterone is a steroid hormone that acts on the endometrium. It is known
for producing physical and mood-related side effects. Few studies have
looked into how progesterone levels affect embryo development and quality.
This study aimed to find a cutoff level for serum progesterone on the day of
HCG administration from which embryo quality is impaired.MethodsThe study included 145 cycles, from which 885 oocytes and 613 embryos were
obtained. All patients had their serum progesterone levels measured on the
day of HCG administration. Data sets were collected from patient medical
records. The chi-square test was used to assess qualitative variables and
the Mann-Whitney test to evaluate quantitative variables.ResultsStatistical analysis revealed that serum progesterone levels and reproductive
variables were not significantly associated. In regards to oocyte maturity,
however, when progesterone levels were greater than 1.3 ng/mL the
probability of oocytes being immature increased by 12.7%. The fragmentation
rate of embryos categorized as "top quality" in D3 increased proportionately
to increases in progesterone levels (12.23%).ConclusionHigh progesterone levels appeared to be correlated with increased embryo
fragmentation rates, but high serum levels of the hormone on the day of HCG
administration had no impact on reproductive variables and were not
associated with impaired embryo development.
This paper reports the case of a patient who sought assisted reproductive
technology (ART) treatment and was referred to pre-implantation genetic
diagnosis (PGD) on account of a chromosomal translocation presented with
secondary infertility. The patient underwent a highly complex ART treatment and
had 14 metaphase II oocytes collected on the day of follicular aspiration. The
embryos were taken to extended culture and five were biopsied and vitrified. The
embryo genetic report showed aneuploidy in four of the blastocysts, while the
other resulted in 46, XX. In conclusion, chromosome translocations involving the
X chromosome might result in the deregulation of gene expression and defective
ovarian formation. Therefore, the genes present in the X chromosome are believed
to be essential in normal ovarian function.
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