A infecção crônica pelo vírus da Hepatite C (HCV) é considerada grave, podendo progredir para cirrose e descompensação hepática. Objetivos: Verificar a prevalência da infecção pelo HCV entre os indivíduos triados, entre setembro de 2007 e setembro de 2009 determinando a correlação dos níveis séricos de anticorpos com presença do RNA viral. Métodos: Os resultados sorológicos da pesquisa de anticorpos anti HCV pelo método imunoenzimático ELISA (BioMérieux®) de 4536 indivíduos foram analisados retrospectivamente. O resultado da pesquisa molecular (COBAS AMPLICOR HCV Roche Diagnostics®) de 79 pacientes foi obtido através de registros médicos, sendo os genótipos 1 e 3 os mais frequentes. A probabilidade do resultado sorológico estar relacionado com a pesquisa molecular foi verificada através de curva ROC, construída para diferentes valores de Índex (DO amostra/DO ponto de corte do ELISA). Resultados: Das amostras para as quais foi realizado o teste molecular, 82,3% confirmaram presença de RNA HCV. Os Valores preditivos positivos (VPP) e Valores preditivos negativos (VPN) para presença de viremia, considerando valores de Índex 4,0 3,0 e 2,0 foram: para Índex 4,0 sensibilidade (S) de 62%, especificidade (E) 64%, VPP de 89%, VPN de 27%. Para Índex 3,0 S=93,0%; E=36,0%, VPP 87%, VPN 55%. Para Índex 2,0 S=100%, E=21%, VPP 85%; VPN 100%. Conclusão: Pode-se inferir que há grande probabilidade de pacientes com reação sorológica para pesquisa de anti-HCV reagente e Índex menor que 2,0, terem suprimido a infecção pelo HCV, e com Índex superior a 4,0 apresentarem viremia presente. ResumoChronic infection with hepatitis C virus (HCV) is a severe infection that may progress to cirrhosis and hepatic decompensation. Objectives: To determine the prevalence of HCV infection amongst screened individuals, between September 2007 and September 2009 to determine the relationships between serum antibody levels and the presence of viral RNA. Methods: Serological results of the research to anti HCV antibodies by enzyme-linked immunosorbent assay-ELISA (BioMérieux ®) of 4536 subjects were analyzed retrospectively. The results of molecular research (Cobas AMPLICOR HCV Roche Diagnostics ®) of 79 patients were extracted from medical records, and genotypes 1 and 3 were the most frequent. The probability of serologic results to be related to molecular analysis was verified by ROC curve constructed for different values of Index (OD sample / OD cutoff ELISA). Results: Of samples for which the molecular test was performed, 82.3% confirmed the presence of HCV RNA. Positive predictive values (PPV) and negative predictive values (NPV) for the presence of viremia, considering Index values of 4.0 3.0 and 2.0 were: for the Index 4.0 sensitivity (S) of 62%, specificity (Sp) 64%, PPV 89%, NPV of 27%. In the Index 3.0 S = 93.0%; E = 36.0%, PPV 87%, NPV 55%. In the Index 2.0% S=100, E=21%, PPV 85%; VPN 100%. Conclusion: It can be inferred that there is high probability of patients with serological reaction for screening anti-HCV reagent, presenting Index lower...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.