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Recebido em 26/2/08; aceito em 10/9/08; publicado na web em 2/2/09 CHEMICAL CONSTITUENTS FROM Sebastiania macrocarpa MUELL. ARG. (EUPHORBIACEAE). The chemical investigation of the methanolic extract of the aerial part of Sebastiania macrocarpa allowed the isolation of the mixture of steroids β-sitosterol and stigmasterol, gallic acid, and scopoletin. The hexane extract of the roots allowed the isolation of the triterpene lupeol and of the macrociclic diterpene (+)-tonantzitlolone. The structures of all compounds isolated were identified on the basis of their spectral data and by comparison of their spectral data with values described in the literature. This is the first report involving the chemical investigation of this species.