The EGF/IGF growth factors are potent mitogens that regulate cell proliferation and cell survival and are involved in prostate cancer development. Using laser microdissection technology and real-time PCR, together with immunohistochemistry, we have explored the growth factor and integrin dependent PI3-kinase/ PTEN/Akt signalling pathway in prostate cell lines and tumour samples by analysing EGF-R, IGF1-R, ILK, b3 integrin, PTEN and p-Akt protein expression. We provide evidence that loss of PTEN expression rather than upregulated EGF/IGF1 receptor expression was responsible for increased p-Akt in neoplastic prostate cells. We therefore compared PTEN expression in patient biopsies at first time diagnosis recruited prospectively (Study I, 112 patients) and patients with confirmed metastasis recruited retrospectively from the Luxembourg cancer registry (Study II, 42 patients). In Study I, loss of PTEN expression at first time diagnosis was found in 26 of 112 patients (23%). In Study II, 25 of the 42 patients (59%) with lymph node metastasis had complete loss of PTEN expression in both the neoplastic glands of the prostate and the invasive prostate cancer cells in the lymph node, and of these 13 (52%) exhibited already loss of PTEN expression at first diagnosis. These findings demonstrate that loss of PTEN expression is an important factor in progression towards metastatic disease and could potentially serve as an early prognostic marker for prostate cancer metastasis. ' 2006 Wiley-Liss, Inc.

Efficient OR capacity utilization and the optimized use of human resources allowed an additional 1820 interventions to be carried out per year without any increase in human resources. In addition, perioperative patient monitoring was increased up to 100%.

No abstract

Review: Der Übersichtsartikel (1 l Literaturhinweise) behandelt die Beseitigung.halogenhaltiger organischer Verbindungen aus Abwassem, u.a. durch Strippen (beispielsweise an Ethylendichlorid), durch Adsorption an A‐Kohle (beispielsweise Aldrin, Dieldrin, DDT und andere wenig flüchtige Verbindungen), durch Adsorption an Polymerharzen (trotz geringer Adsorptionskapazität ist infolge der hoheren Dichte die Volumenkapazität mit der von A‐Kohle vergleichbar), durch biologische Oxidation (mittels mutierten Pseudomonasarten bei 30°C an chlorierten Benzolen und Phenolen ist die Zeitabhängigkeit der Ringspaltung angefuhrä), durchExtraktion.