ILK as a potential marker gene to ascertain specific adenocarcinoma cell mRNA isolation from frozen prostate biopsy tissue sections

Complete loss of PTEN expression as a possible early prognostic marker for prostate cancer metastasis

“…22 Briefly, the primer pairs and probes for ILK, EGF-R, IGF1-R and b3 integrin mRNA quantification were customdesigned by Applied Biosystems, while the primer pairs for quantification of the reference 18S ribosomal RNA gene were purchased from the Applied Biosystems catalogue. For each real-time PCR reaction, 2.5-10 ll of patient cDNA was used (based on bactin amplification by standard PCR) from the 100 ll Superscript FIGURE 1 -Central regulatory role of PTEN in the growth factor and integrin dependent PI3-kinase/PTEN/Akt signalling pathway.…”

“…22 For each patient, one slide was stained with haematoxylin-eosin (H&E) by standard methods to allow the interpretation of the detailed histology and the tumour composition by light microscopy. For LMD processing, the slides were stained with H&E as described, 22 and prostatic epithelial cells of the tissue sections were selectively microdissected using a Leica AS LMD microscope (Leica, Wetzlar, Germany).…”

“…20,21 We have previously shown that laser microdissection (LMD) of adenocarcinoma cells from frozen prostate tissue sections, combined with quantitative real-time PCR analysis of mRNA expression, is a reliable method to investigate phenotypic changes that differentiate prostate adenocarcinoma cells from normal prostate epithelial cells. 22 Here we have applied this technology to precisely quantify IGF-1 and EGF receptor expression, together with ILK and b3 integrin protein expression in adenocarcinoma cells isolated from prostate needle biopsy tissue sections. In addition, we have correlated these results with PTEN expression in a retrospective study of 42 prostate cancer patients with lymph node metastasis, as well as in a prospective study of 127 patients who underwent first time prostate biopsy and were diagnosed positive for prostate adenocarcinoma.…”

Integrin-linked Kinase Functions as a Tumor Promoter in Bladder Transitional Cell Carcinoma

“…Integrin-linked kinase (ILK) is a molecular target which can lead to various oncogenic-related events including cell migration, invasion and an inhibition of apoptosis. Increased ILK activity is correlated to several varieties malignant tumors including prostate (Kieffer et al, 2005), colon (Marotta et al, 2003), gastric (Ito et al, 2003), and so on. ILK expression was significantly associated with tumor grade, T status, lymph node metastasis and stage (Graff et al, 2001;Takanami, 2005), and tumor angiogenesis (Tan et al, 2004).…”

“…A series of studies had found that ILK abnormally over-expressed and was activated in prostate cancer (Kieffer et al, 2005), ovarian cancer (Ahmed et al, 2003), colon cancer (Marotta et al, 2003), gastric cancer (Ito et al, 2003) and other multi-malignant tumors, ulitizing many mechanism: such as inhibiting cell apoptosis, and promoting cell proliferation, these changes move forward a single step to affect biological function (Gu et al, 2012), integrin and other element enhance tumor tissue invasion force through the endothelial cells of the adhesion strength changes (Kaiser et al, 2012). But the expression of ILK in the BTCC and its role in occurrence and development of BTCC are still unclear.…”