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Glacier-fed streams (GFSs) are extreme and rapidly vanishing ecosystems, and yet they harbor diverse microbial communities. Although our understanding of the GFS microbiome has recently increased, we do not know which microbial clades are ecologically successful in these ecosystems, nor do we understand potentially underlying mechanisms. Ecologically successful clades should be more prevalent across GFSs compared to other clades, which should be reflected as clade-wise distinctly low phylogenetic turnover. However, methods to assess such patterns are currently missing. Here we developed and applied a novel analytical framework, “phyloscore analysis”, to identify clades with lower spatial phylogenetic turnover than other clades in the sediment microbiome across twenty GFSs in New Zealand. These clades constituted up to 44% and 64% of community α-diversity and abundance, respectively. Furthermore, both their α-diversity and abundance increased as sediment chlorophyll a decreased, corroborating their ecological success in GFS habitats largely devoid of primary production. These clades also contained elevated levels of putative microdiversity than others, which could potentially explain their high prevalence in GFSs. This hitherto unknown microdiversity may be threatened as glaciers shrink, urging towards further genomic and functional exploration of the GFS microbiome.

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In glacier-fed streams, ecological windows of opportunity allow complex microbial biofilms to develop and transiently form the basis of the food web, thereby controlling key ecosystem processes. Using metagenome-assembled genomes, we unravel strategies that allow biofilms to seize this opportunity in an ecosystem otherwise characterized by harsh environmental conditions. We observe a diverse microbiome spanning the entire tree of life including a rich virome. Various co-existing energy acquisition pathways point to diverse niches and the exploitation of available resources, likely fostering the establishment of complex biofilms during windows of opportunity. The wide occurrence of rhodopsins, besides chlorophyll, highlights the role of solar energy capture in these biofilms while internal carbon and nutrient cycling between photoautotrophs and heterotrophs may help overcome constraints imposed by oligotrophy in these habitats. Mechanisms potentially protecting bacteria against low temperatures and high UV-radiation are also revealed and the selective pressure of this environment is further highlighted by a phylogenomic analysis differentiating important components of the glacier-fed stream microbiome from other ecosystems. Our findings reveal key genomic underpinnings of adaptive traits contributing to the success of complex biofilms to exploit environmental opportunities in glacier-fed streams, which are now rapidly changing owing to global warming.

Glacier-fed streams (GFSs) exhibit near-freezing temperatures, variable flows, and often high turbidities. Currently, the rapid shrinkage of mountain glaciers is altering the delivery of meltwater, solutes, and particulate matter to GFSs, with unknown consequences for their ecology. Benthic biofilms dominate microbial life in GFSs, and play a major role in their biogeochemical cycling. Mineralization is likely an important process for microbes to meet elemental budgets in these systems due to commonly oligotrophic conditions, and extracellular enzymes retained within the biofilm enable the degradation of organic matter and acquisition of carbon (C), nitrogen (N), and phosphorus (P). The measurement and comparison of these extracellular enzyme activities (EEA) can in turn provide insight into microbial elemental acquisition effort relative to environmental availability. To better understand how benthic biofilm communities meet resource demands, and how this might shift as glaciers vanish under climate change, we investigated biofilm EEA in 20 GFSs varying in glacier influence from New Zealand’s Southern Alps. Using turbidity and distance to the glacier snout normalized for glacier size as proxies for glacier influence, we found that bacterial abundance (BA), chlorophyll a (Chl a), extracellular polymeric substances (EPS), and total EEA per gram of sediment increased with decreasing glacier influence. Yet, when normalized by BA, EPS decreased with decreasing glacier influence, Chl a still increased, and there was no relationship with total EEA. Based on EEA ratios, we found that the majority of GFS microbial communities were N-limited, with a few streams of different underlying bedrock geology exhibiting P-limitation. Cell-specific C-acquiring EEA was positively related to the ratio of Chl a to BA, presumably reflecting the utilization of algal exudates. Meanwhile, cell-specific N-acquiring EEA were positively correlated with the concentration of dissolved inorganic nitrogen (DIN), and both N- and P-acquiring EEA increased with greater cell-specific EPS. Overall, our results reveal greater glacier influence to be negatively related to GFS biofilm biomass parameters, and generally associated with greater microbial N demand. These results help to illuminate the ecology of GFS biofilms, along with their biogeochemical response to a shifting habitat template with ongoing climate change.

International audienceThis study investigates the possibility of Holocene glaciation on Mount Olympus (Greece) with a respective local temperature–precipitation equilibrium line altitude (TP-ELA) at c. 2200m a.s.l., based on geomorphologic and paleoclimatic evidence. At present, the local TP-ELA is situated above the mountain’s summit (c. 2918 m a.s.l.), but permanent snowfields and ice bodies survive within Megala Kazania cirque between c. 2400 and c. 2300 m a.s.l., because of the cirque’s maritime setting that results from its close proximity (c. 18km) to the Aegean Sea and of the local topographical controls. The snow and ice bodies occupied a considerably larger area and attained a stabilization phase between AD 1960 and 1980, also manifested from aerial photographs, a period characterized by increased winter precipitation (Pw) with subsequent TP-ELA depression to c. 2410m a.s.l. Mid- to late-20th- century Pw and TP-ELA variations exhibit negative correlations with the winter North Atlantic Oscillation index (NAOw) at annual and multidecadal (30 years) timescales. Late Holocene (AD 1680–1860) reconstructed summer mean temperatures were lower by Ts < 1.1°C in relation to the reference period between AD 1960 and 1980 and were also superimposed to negative NAOw phases, thus bracketing this time interval as a favorable one to glacial formation and/or advance. Millennial-scale annual precipitation reconstructions at the hypothesized TP-ELA (c. 2200m a.s.l.) point the period between 8 and 4 kyr BP as another glacier-friendly candidate. The mid-Holocene rather simplistic sequence of potential glacial advance phase was disturbed by short-lived cold climatic deteriorations, well-documented over the northern Aegean region that may partly explain the multicrested shape of the highest (c. 2200 m a.s.l.) morainic complex of Megala Kazania cirque

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Cone penetration testing has been widely used since the 1950s for determining the subsurface geotechnical conditions of unconsolidated sediments. This paper highlights the potential value of cone penetration testing as an aid to define the stratigraphic structure of Holocene sedimentary deposits. By calibrating cone penetration test logs with adjacent borehole logs and by utilizing all the available information produced during geotechnical surveying, stratigraphic models that accurately describe the vertical and lateral boundaries, as well as the stacking pattern, of Late Quaternary systems can be constructed. The widespread application and technical simplicity of cone penetration testing, combined with simple data interpretation via correlation with adjacent borehole logs, yield a useful and inexpensive tool for sedimentological investigations. This methodology is illustrated using data from 36 cone penetration tests and 11 boreholes on the Holocene deltaic plain of the Aliakmon River, Greece. Sedimentological and stratigraphic information from core log correlations, the spatial distribution of cone penetration test parameters, sediment grain size and per cent concentration of organic matter are utilized. The results suggest, that in sequence stratigraphic terms, the delta is divided into a lowstand systems tract composed by fluvial gravels and sands (U0) of Late Pleistocene age, as well as from red oxidized clays, and a transgressive systems tract represented by fluvial channel sands (U1), overlain by a thin transgressive sand sheet of coastal origin (U2), characterized by fining upward trends. The highstand systems tract is constituted by a variety of stratigraphic units (U3 to U7) and depositional environments, characterized by coarsening upward sequences, representing both aggradational and progradational facies, and dominated by the presence of three prograding wedges. Detailed definition of the thickness, vertical boundaries and stacking pattern of the resolved stratigraphic units, presented as a two-dimensional stratigraphic model, demonstrates the applicability of the proposed method.