Abstract:Monitoring the dynamics in wheat crops requires near-term observations with high spatial resolution due to the complex factors influencing wheat growth variability. We studied the prospects for monitoring the biophysical parameters and nitrogen status in wheat crops with low-cost imagery acquired from unmanned aerial vehicles (UAV) over an 11 ha field. Flight missions were conducted at approximately 50 m in altitude with a commercial copter and camera system-three missions were performed between booting and maturing of the wheat plants and one mission after tillage. Ultra-high resolution orthoimages of 1.2 cm·px −1 and surface models were generated for each mission from the standard red, green and blue (RGB) aerial images. The image variables were extracted from image tone and surface models, e.g., RGB ratios, crop coverage and plant height. During each mission, 20 plots within the wheat canopy with 1 × 1 m 2 sample support were selected in the field, and the leaf area index, plant height, fresh and dry biomass and nitrogen concentrations were measured. From the generated UAV imagery, we were able to follow the changes in early senescence at the individual plant level in the wheat crops. Changes in the pattern of the wheat canopy varied drastically from one mission to the next, which supported the need for instantaneous observations, as delivered by UAV imagery. The correlations between the biophysical parameters and image variables were highly significant during each mission, and the regression models calculated with the principal components of the image variables yielded R 2 values between 0.70 and 0.97. In contrast, the models of the nitrogen concentrations yielded low R 2 values with the best model obtained at flowering (R 2 = 0.65). The nitrogen nutrition index was calculated with an accuracy of 0.10 to 0.11 NNI for each mission. For all models, information about the surface models and image tone was important. We conclude that low-cost RGB UAV imagery will strongly aid farmers in observing biophysical characteristics, but it is limited for observing the nitrogen status within wheat crops.

Due to its perennial nature and size, the acquisition of phenotypic data in grapevine research is almost exclusively restricted to the field and done by visual estimation. This kind of evaluation procedure is limited by time, cost and the subjectivity of records. As a consequence, objectivity, automation and more precision of phenotypic data evaluation are needed to increase the number of samples, manage grapevine repositories, enable genetic research of new phenotypic traits and, therefore, increase the efficiency in plant research. In the present study, an automated field phenotyping pipeline was setup and applied in a plot of genetic resources. The application of the PHENObot allows image acquisition from at least 250 individual grapevines per hour directly in the field without user interaction. Data management is handled by a database (IMAGEdata). The automatic image analysis tool BIVcolor (Berries in Vineyards-color) permitted the collection of precise phenotypic data of two important fruit traits, berry size and color, within a large set of plants. The application of the PHENObot represents an automated tool for high-throughput sampling of image data in the field. The automated analysis of these images facilitates the generation of objective and precise phenotypic data on a larger scale.

Weed detection with aerial images is a great challenge to generate field maps for site-specific plant protection application. The requirements might be met with low altitude flights of unmanned aerial vehicles (UAV), to provide adequate ground resolutions for differentiating even single weeds accurately. The following study proposed and tested an image classifier based on a Bag of Visual Words (BoVW) framework for mapping weed species, using a small unmanned aircraft system (UAS) with a commercial camera on board, at low flying altitudes. The image classifier was trained with support vector machines after building a visual dictionary of local features from many collected UAS images. A window-based processing of the models was used for mapping the weed occurrences in the UAS imagery. The UAS flight campaign was carried out over a weed infested wheat field, and images were acquired between a 1 and 6 m flight altitude. From the UAS images, 25,452 weed plants were annotated on species level, along with wheat and soil as background classes for training and validation of the models. The results showed that the BoVW model allowed the discrimination of single plants with high accuracy for Matricaria recutita L. (88.60%), Papaver rhoeas L. (89.08%), Viola arvensis M. (87.93%), and winter wheat (94.09%), within the generated maps. Regarding site specific weed control, the classified UAS images would enable the selection of the right herbicide based on the distribution of the predicted weed species.

The non-invasive detection of chilling injury (CI) symptoms in banana may potentially be approached by means of monitoring changes in the pigment contents and texture of the exocarp. In the present study, laser diodes emitting at 660 and 785 nm were applied to acquire images of backscattered light from intact banana fruits. The idea was to monitor chlorophyll and texture changes by means of relevant wavelengths, respectively. Bananas were stored for 2 days at 13 °C (control), 6 °C (chilling temperature), and subsequently 1 day at ambient temperature to allow the symptom development. Parameters obtained from the backscattering images and their combinations were applied for detecting chilling injury. Significant (P < 0.05) interaction of backscattering properties and treatment factors (temperature, ripening stage, and treatment time) were found. Classification of control and chill-injured samples in ripe fruits measured at 660 nm and 785 nm resulted in misclassification error as low as 6% and 8% for early detection, and 0.67% and 1.33% for detection after storage, respectively. The physiological relevance of the variation measured at the two wavelengths was pointed out by means of destructive pigment and water analyses.

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In apple cultivation, spatial information about phenotypic characteristics of tree walls would be beneficial for precise orchard management. Unmanned aerial vehicles (UAVs) can collect 3D structural information of ground surface objects at high resolution in a cost-effective and versatile way by using photogrammetry. The aim of this study is to delineate tree wall height information in an apple orchard applying a low-altitude flight pattern specifically designed for UAVs. This flight pattern implies small distances between the camera sensor and the tree walls when the camera is positioned in an oblique view toward the trees. In this way, it is assured that the depicted tree crown wall area will be largely covered with a larger ground sampling distance than that recorded from a nadir perspective, especially regarding the lower crown sections. Overlapping oblique view images were used to estimate 3D point cloud models by applying structure-from-motion (SfM) methods to calculate tree wall heights from them. The resulting height models were compared with ground-based light detection and ranging (LiDAR) data as reference. It was shown that the tree wall profiles from the UAV point clouds were strongly correlated with the LiDAR point clouds of two years (2018: R2 = 0.83; 2019: R2 = 0.88). However, underestimation of tree wall heights was detected with mean deviations of −0.11 m and −0.18 m for 2018 and 2019, respectively. This is attributed to the weaknesses of the UAV point clouds in resolving the very fine shoots of apple trees. Therefore, the shown approach is suitable for precise orchard management, but it underestimated vertical tree wall expanses, and widened tree gaps need to be accounted for.

When using spectrophotometric transmittance readings of fruit extracts, the analysis of single carotenoids is difficult because of coinciding absorption bands of the various carotenoids and chlorophylls present in the solution. Aimed at the separate analyses of pigments, an iteratively applied linear regression was developed based on spectral profiles of pigment standards. The iterative approach was validated by dilution series of pigments and compared with commonly applied equation systems. High coefficients of determination and low measuring uncertainties were found for chlorophyll a and b (R(2) > or = 0.99, root mean square error RMSE < or = 10%). Carotenoids were separately analyzed with R(2) = 0.99, R(2) = 0.96, and R(2) = 0.98 for lycopene, beta-carotene, and lutein, respectively. The approach based on the spectral profiles provided low measuring uncertainties even if lutein was additionally present in the solutions, which was not possible with common data analyses. Subjecting tomato tissues (Solanum lycopersicum L.) to the iterative approach, contents of in vivo measured pigments were calculated with R(2) = 0.82, R(2) = 0.84, R(2) = 0.67, and R(2) = 0.03 for chlorophyll a and b, lycopene, and beta-carotene, respectively.

a b s t r a c tIn high-value sweet cherry (Prunus avium), the red coloration -determined by the anthocyanins content -is correlated with the fruit ripeness stage and market value. Non-destructive spectroscopy has been introduced in practice and may be utilized as a tool to assess the fruit pigments in the supply chain processes. From the fruit spectrum in the visible (Vis) wavelength range, the pigment contents are analyzed separately at their specific absorbance wavelengths.A drawback of the method is the need for re-calibration due to varying optical properties of the fruit tissue. In order to correct for the scattering differences, most often the spectral intensity in the visible spectrum is normalized by wavelengths in the near infrared (NIR) range, or pre-processing methods are applied in multivariate calibrations.In the present study, the influence of the fruit scattering properties on the Vis/NIR fruit spectrum were corrected by the effective pathlength in the fruit tissue obtained from time-resolved readings of the distribution of time-of-flight (DTOF). Pigment analysis was carried out according to Lambert-Beer law, considering fruit spectral intensities, effective pathlength, and refractive index. Results were compared to commonly applied linear color and multivariate partial least squares (PLS) regression analysis. The approaches were validated on fruits at different ripeness stages, providing variation in the scattering coefficient and refractive index exceeding the calibration sample set.In the validation, the measuring uncertainty of non-destructively analyzing fruits with Vis/NIR spectra by means of PLS or Lambert-Beer in comparison with combined application of Vis/NIR spectroscopy and DTOF measurements showed a dramatic bias reduction as well as enhanced coefficients of determination when using both, the spectral intensities and apparent information on the scattering influence by means of DTOF readings. Corrections for the refractive index did not render improved results.