solvent program, at a flow rate of 1 mL/minute, was 5 min isocratic, 15 min linear increase in water from 0.5 to 1.4, and 20 min isocratic at the final solvent composition of 6:8:1.4. We achieved excellent separation (Figures 1 and 2) in 10 min less time beginning with a solvent composition of 6:8:0.5 and running a linear increase in water to a final composition of 6:8:1.5, flow rate 1.5 mL/min.The methods for phospholipids of red blood cell ghosts and squid axions did not separate sufficient quantities for further analysis. The data in Table I show that our method separates sufficient quantities of the phospholipids
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