ABSTRACT:The surface modification of monodispersecrosslinked polymeric microspheres was carried out by introducing hydroxyl groups on the surface and utilizing the redox initiation system. The emulsions of the second monomer mixture were swollen into the monodisperse PS seed particles. The hydroxyl groups were introduced by hydrolysis of the acetate groups on the surface of microspheres. Ceric ammonium sulfate in sulfuric acid solution was employed to graft the acrylic monomer onto the polymeric microspheres. The surface characteristics of the surfacemodified particles were confirmed by FTIR, SEM, and TGA measurements. From the FE-TEM image, a uniform coating layer was confirmed on the surface of microsphere. In DSC analysis, only an exothermal peak appeared when high content of DVB was used in the seeded polymerization, while, T g s emerged after hydrolysis and graft polymerization using the low content of DVB in the second monomer.
Summary: This communication describes an enzyme stabilization method that allows the use of enzymes irrespective of environmental factors, especially heat, while maintaining their activity for a long time. We have designed enzyme microcapsules that consist of papain enzyme cores, poly(propylene glycol) interlayers, and poly(ε‐caprolactone) walls. By confocal laser scanning microscopy measurements and the thermal stability of papain‐loaded microcapsules, it is demonstrated that the papain is surrounded by a hydrophobic polyol layer and stabilized by the exclusive volume effect. In our study, improved thermal stability can be obtained by using more hydrophobic long‐chained polyols, which is understood to be attributed to the effective formation of a hydrophobic polyol layer between the papain and the polymer wall by means of conformational anchoring in the interface.(A) A CLSM image of a PCL microcapsule containing FITC‐labeled papain and RBITC‐labeled PPG at the same time. (B) A scheme of the role of hydrophobic polyols in the interface of enzyme and polymer.magnified image(A) A CLSM image of a PCL microcapsule containing FITC‐labeled papain and RBITC‐labeled PPG at the same time. (B) A scheme of the role of hydrophobic polyols in the interface of enzyme and polymer.
Objectives : This study aimed to provide scientific information on the characteristics of organic matters in influents and effluents of sewage treatment plants (STPs) in Gyeongbuk province, Korea, for better performance of treatment processes in the plants.Methods : We selected six STPs with each capacity over 30,000 mZ3/day in Gyeongbuk province, and analyzed water quality data in influents and effluents of the plants from 2013 to 2020. Also, the removal efficiencies of pollutants were assessed with the operational data. In 2020, characteristics and origins of dissolved organic matters (DOM) in influents and effluents were investigated using the fluorescence excitation emission matrix (FEEM) analysis.Results and Discussion : The average BOD5/CODMn ratios of influents and effluents from the STPs were 1.60 and 0.27, respectively. High variability in BOD5/CODMn ratios was observed for both influents and effluents. The CODMn/TOC ratios of the influents and effluents were the same with an average of 1.6. Although the biological treatment process was different for each STP, the organic matter removal efficiency was similar, and the TOC removal efficiency was 86.5~91.7%. The representative spectra of DOM identified through FEEM analysis was peak C (humic-acid like substance) region, and under the same carbon concentration (2 mg-C/L) the fluorescence intensity of effluents was stronger than influents possibly due to the influence of soluble microbial products (SMP). It was found that DOM of both influent and effluent originated from microorganisms, and the difference in water quality of DOM was statistically significant.Conclusions : The characteristics and fate of organic matters in influents and effluents of the six STPs were similar regardless of plant location. The results of this study can be used as basic information to efficiently control organic matters in the STPs.
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