Forests play a key role in climate change mitigation through sequestering and storing carbon dioxide from the atmosphere. However, there is inadequate information about carbon accumulation and sequestered by community reserved forests in Tanzania. A study was carried to quantify the amount of carbon sequestered in two forests namely; Nyasamba and Bubinza of Kishapu district, northwestern Tanzania. A ground-based field survey design under a systematic sampling technique was adopted. A total of 45 circular plots (15 m radius) along transects were established. The distances between transect and plots were maintained at 550 and 300 m, respectively. Data on herbaceous C stocking potential was determined using destructive harvest method while tree carbon stocking was estimated by allometric equations. The collected data were organized on excel datasheet followed by descriptive analysis for quantitative information using Computer Microsoft Excel and SPSS software version 20, while soil samples were analyzed based on the standard laboratory procedures. Results revealed higher carbon sequestration of 102.49±39.87 and 117.52±10.27 for soil pools than plants both herbaceous (3.01±1.12 and 6.27±3.79 t CO2e/yr) and trees (5.70±3.15 and 6.60±2.88 t CO2e/yr) for Nyasamba and Bubinza respectively. The study recorded a potential variation of soil carbon sequestration, which varied across depths category (P < 0.05). However, there was no difference across sites (P >0.05) and species (P > 0.05) for herbaceous and trees. The findings of this study portrayed a significantly low value for carbon stocking and sequestration potential for enhanced climate change mitigation. Therefore, proper management of community reserved forest is required to accumulate more C for enhancing stocking potential hence climate change mitigation through CO2 sequestration offsets mechanism.
The present study aimed to determine
in vitro
antimicrobial activities and phytochemical analysis of
Harrisonia abyssinica
and
Vepris simplifolia
which are commonly used as traditional medicine in Tanzania. Sensitivity of bacterial and fungal species against plants extracts were determined using serial microdilution method. In this method, the lowest inhibitory concentration which prevented microbial growth considered as minimum inhibitory concentration (MIC). The study also evaluated phytochemical compounds present in the leaf, stem and root barks of
H. abyssinica
. It was revealed that eight extracts from
H. abyssinica
inhibited growth of three bacteria namely
Staphylococcus aureus
,
Enterococcus faecium
and
Streptococcus uberis
at MIC value less than 1 mg/mL. It was further revealed that, three extracts from
V. simplifolia
exhibited high antibacterial and antifungal activity. The preliminary phytochemical analysis revealed presence of various phytochemicals namely alkaloids, terpenoid, flavonoid, tannin and saponin. It was concluded that presence of large number of phytochemicals in the plant extracts may be associated with pharmacological properties of
H. abyssinica
and
V. simplifolia
and therefore this study provide alternative to synthetic antimicrobial agents.
Background: Medicinal plants have been of great value to human healthcare in most parts of the world for thousands of years. In Tanzania, over 12,000 species of higher plants have been reported, and about 10% are estimated to be used as medicines to treat different human health conditions. The present study aimed to determine in vitro antimicrobial activities and phytochemical analysis of Ormocarpum trichocarpum and Euclea divinorum which are commonly used as a traditional medicine in Tanzania.
Methods: Minimum Inhibitory Concentration (MIC) of plants extracts against tested bacterial and fungal species were determined using 96 wells microdilution method. In this method, 50 μL of nutrient and saboraud’s dextrose broth for bacteria and fungus respectively were loaded in each well followed by 50 μL of extract to make final volume of 100 μL. Subsequently 50 μL were transferred from first rows of each well to the second rows and the process was repeated down the columns to the last wells from which 50 μL were discarded. Thereafter, 50 μL of the selected bacterial and fungal suspension was added to each well thus making final volume of 100μL. The lowest concentration which showed no microbe growth was considered as MIC. The study also evaluated phytochemical compounds present in the ethyl acetate extracts from O. trichocarpum stem bark and E. divinorum root bark extract using Gas Chromatography-Mass Spectrometry (GC-MS) technique.
Results: It was revealed that 66% of the tested microbes were susceptible to plant extracts at MIC value of 0.39 mg/mL whereas 83% being susceptible to extracts at MIC value of 0.781 mg/mL. Interestingly, four out of 18 tested plant extracts exhibited high antifungal activity below that of the standard antifungal drug, fluconazole. The GC-MS analysis revealed the presence of various low molecular weight phytochemicals which belongs to six groups of secondary metabolites namely dieterpenes, alphatic hydrocarbons, tetraterpenes, sesquiterpenes, steroid and triterpenes.
Conclusion: It was concluded that the presence of various phytochemicals in the tested plant extracts may be associated with pharmacological properties of O. trichocarpum and E. divinorum and therefore justifying ethnomedical usage of such plants.
Keywords: Antibacterial, antifungal, Ormocarpum trichocarpum, Euclea divinorum
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