Antimicrobial activities and phytochemical analysis of Harrisonia abyssinica (Oliv) and Vepris simplicifolia (Verd) extracts used as traditional medicine in Tanzania

Abstract

The present study aimed to determine in vitro antimicrobial activities and phytochemical analysis of Harrisonia abyssinica and Vepris simplifolia which are commonly used as traditional medicine in Tanzania. Sensitivity of bacterial and fungal species against plants extracts were determined using serial microdilution method. In this method, the lowest inhibitory concentration which prevented microbial growth considered as minimum inhibitory concentration (MIC). The study also evaluated phytochemical compounds present in the leaf, stem and root barks of H. abyssinica . It was revealed that eight extracts from H. abyssinica inhibited growth of three bacteria namely Staphylococcus aureus , Enterococcus faecium and Streptococcus uberis at MIC value less than 1 mg/mL. It was further revealed that, three extracts from V. simplifolia exhibited high antibacterial and antifungal activity. The preliminary phytochemical analysis revealed presence of various phytochemicals namely alkaloids, terpenoid, flavonoid, tannin and saponin. It was concluded that presence of large number of phytochemicals in the plant extracts may be associated with pharmacological properties of H. abyssinica and V. simplifolia and therefore this study provide alternative to synthetic antimicrobial agents.

Medicinal plants used for cutaneous wound healing in Uganda; ethnomedicinal reports and pharmacological evidences

“…The present review retrieved and compiled ethnomedicinal and ethnopharmacological information of these wound healing plants (165 species). Of the 165 species, only fifty-four (33 %) of the plants have been experimentally validated for their wound healing activities in different pharmacological, phytochemical, and toxicological studies, including [ [143] , [144] , [145] , [146] , [147] , [148] , [149] , [150] , [151] , [152] , [153] , [154] , [155] , [156] , [157] , [158] , [159] , [160] , [161] , [162] , [163] , [164] , [165] , [166] , [167] , [168] , [169] , [170] , [171] , [172] , [173] , [174] , [175] , [176] , [177] , [178] , [179] , [180] , [181] , [182] , [183] , [184] , [185] , [186] , [187] , [188] , [189] , [190] , [191] , [192] , [193] , [194] , [195] , [196] , [197] , [198] , [199] , [200] , [201] , [202] , [203] , [204] , [205] , …”

Antibacterial activity and phytochemical screening of traditional medicinal plants most preferred for treating infectious diseases in Habru District, North Wollo Zone, Amhara Region, Ethiopia

“…Phytochemical screening was conducted on both ethanol and aqueous extracts using the methods outlined by [ 26 , 30 – 32 ] to determine the presence of secondary metabolites. Preliminary phytochemical screening of the S .…”

“…Phytochemical screening was conducted on both ethanol and aqueous extracts using the methods outlined by [26,[30][31][32] to determine the presence of secondary metabolites. Preliminary phytochemical screening of the S. somalense, V. sinaiticum, R. nervosus, W. somnifera and C. aurea leaf aqueous extracts were tested for steroid, alkaloid, flavonoid, saponin, tannin, phenol, cardiac glycosides, terpenoid, coumarin, anthraquinine and anthocyanins.…”

Bioactive compounds, antioxidant and antibacterial properties of the pulp, peel and aril of the fruit of Clusia quadrangula (Clusiaceae)

“…The Minimum inhibition concentration of extract for each bacterium was determined as the concentration of the methanolic extract that presented an inhibition percentage greater than 90% (MIC 90) (Dzugan et al, 2020) and was confirmed by the addition of 10 µl and 10 µl iodonitrotetrazolium chloride followed by incubation at 32 °C for 1 h (Kilonzo and Munisi, 2021). The test was performed in triplicate.…”