Live anthrax vaccine containing spores from attenuated strains STI-1 of Bacillus anthracis is used in Russia and former CIS (Commonwealth of Independent States) to prevent anthrax. In this paper we studied the duration of circulation of antibodies specific to spore antigens, the protective antigen (PA), the lethal factor (LF) and their domains (D) in donors’ blood at different times after their immunization with live anthrax vaccine. The relationship between the toxin neutralization activity level and the level of antibodies to PA, LF and their domains was tested. The effect of age, gender and number of vaccinations on the level of adaptive post-vaccination immune response has been studied. It was shown that antibodies against PA-D1 circulate in the blood of donors for 1 year or more after immunization with live anthrax vaccine. Antibodies against all domains of LF and PA-D4 were detected in 11 months after vaccination. Antibodies against the spores were detected in 8 months after vaccination. A moderate positive correlation was found between the titers of antibodies to PA, LF, or their domains, and the TNA of the samples of blood serum from the donors.
A novel approach to the study of erythrocytes instability based on assessment fusability of their plasma membrane is presented. The fusability of erythrocytes normal blood, stored for 7 days at 4˚C, and type 2 patients with diabetes were studied. Human erythrocytes were fused by incubation with La 3+ at final concentrations from 50 to 200 µM. Erythrocytes aggregates were incubated at 37˚C for 120 min. The fusability of erythrocytes was evaluated using a light microscope. It was shown that La 3+ induced extensive fusion of erythrocytes after 7 days of blood storage and erythrocytes of patients with diabetes. Incubation of normal erythrocytes at 37˚C for 120 min does not induce cell fusion.
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