The objective of this study was to evaluate the effects of heat processing on the antifungal activity of honey against Aspergillus niger. A sample of eucalyptus honey was divided into four portions of 250 g each. One of the four honey portions was not heated (Room temperature: 25°C), The other portions were placed in water bath during 24 hours at 40°C, 60°C and 80°C temperatures. The HMF rates, Acidity, pH and the refraction index were determined by harmonized methods. The antifungal tests (Minimum Inhibitory Concentration) were carried out on Sabouraud agar medium embedded with honey according to dilution test. The moisture shows values of 15.65% and 15.83%, pH between 4.10 and 4.24, the free acidity ranges between 33.8 and 38.36 meq kg -1 , Hydroxymethylfurfural (HMF) content shows values between 28.8 and 103.44 mg kg -1 .The antifungal action of the non-heated fraction (Fc) of honey in vitro was marked 46 % (vol/vol) than heated fractions of honey (47%, 48%, and 50%) vol/vol. respectively the antifungal activity of each fraction decreased in the following order: 46% (F25°C), 47% (F40°C), 48% (F60°C) and 50% (F80°C). Our findings indicate that different levels of parameters physical-chemical properties of honey to different temperatures showed inhibitory activity against A.niger with variable degrees but heating honey diminish its antifungal capacity.
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