The objectives of the present study were to investigate strain identification of Echinococcus granulosus infecting camel and human in Qalyubia, Egypt. Therefore partial sequences were generated after gel purification of nested PCR amplified products of mitochondrial NADH 1gene of Echinococcus granulosus complex. Sequences were further examined by sequence analysis and subsequent phylogeny to compare these sequences to those from known strains of E.granulosus circulating globally and retrieved from GenBank. All isolates are homologous to the camel strain, E. canadensis (G6) genotype. Nucleotide mutations generate polymorphism at position of 275 nucleotide, where a thymine replaced a cytosine and at the levels of 385 and 386 nucleotides, where two cytosine substituted a guanine and a thymine respectively. KF815488 Egypt showed typical identity (99.5%) with JN637176 Sudan, HM853659 Iran, AF386533 France and AJ237637 Poland with 0.5% diversion.. Phylogenetic analysis showed a robust tree clustering all isolates with sequences belonging to the camel genotype (G6) variant with strong bootstrap values at relevant nodes and the evolutionary distance between groups is very short. There are two mutations in the sequences of amino acids at the position of 92, where an Alanine is changed to a Valine and at the position of 129, where a Valine is transformed to a Proline. Our record of a single genotype determined a strain which could be incriminated for camel and human infectivity and responsible for its persistence in the endemic areas. Such epidemiological data could guide the application of efficient control strategies of hydatidosis in Egypt.

This investigation is performed on 100 cattle in Kaliobia governorate Egypt aged from 1-6years severity of illness increase with age , these animals suffered from fever (41°C) enlargement lymph node and drop in milk yield emaciation in progressive stages , cattle producers first notice the anemic anaplasmosisinfected animal when it becomes weak and lag behind the herd when these animals were subjected to microscopic examination the degree of parasitaema was recorded as the percentage of infected red blood cells in each blood smear 100 microscopic field wear examined .We report the detection of anaplasma marginale by PCR in blood samples obtained from cattle supposed to be infected. The assay employs primers specific for the gene encoding anaplasma marginale specific PCR using primers derived from msp5 gene .The PCR products for 26 positive samples were subjected to sequence (Labtechnology, Egypt) and BLAST analysis was used for identification of the genomic DNA of these parasites. changes associated with anaplasma marginale in these cattle particular emphasis to the oxidative stress the reduce TAC level may reflect adecrease in antioxidant capacity and CBC change. Blood collected from all animals on EDTA to microscopic examination and PCR to determine type of anaplasma .

Babesia bigemina plays an important role in causing liver and kidney dysfunction in affected animals. The aim of this study evaluating the toxic effect of babesiosis on liver and kidney by measuring biochemical parameters and pathological tissue changes in infected animals with B. Bigemina and chose the best method of treatment. A total of 40 cattle age 1-3 years 30 were suffered from increase in temperatures, off food, Hemoglobin urea, red water from endemic area with babesia. Take sample from infected animals & examine it with microscopic examination found babesia bigemina and confirm by inoculation of heparinized blood from this animals in rats and found rats death at fifth day from infection and histopathological exam found babesia in piroplasma form in pathological tissue of rat's liver & kidney. The infected animals were divided into three groups each group 10 animals untreated group use as control positive and treated groups divided into two groups first group treated with imidocarb only and the second group use imidocarb and lincomcyn as antibiotic drug and compare between two groups by measuring liver& kidney function improvement and compare two groups with negative and positive group which method of treatment more effective in treatment of babesia & decrease toxic effect of babesia.

This investigation is performed on 100 buffaloes native Breed in Kaliobia governorate Egypt aged from 1-3years, these animals suffered from fever (41°C) enlargement lymph node, inappetence, pale mucous membrane, increase lacrimation, emaciation and corneal opacity in progressive stages when these animals were subjected to microscopic examination the degree of parasitaema was recorded as the percentage of infected red blood cells in 1000red blood cell counted .We report the detection of Theileria annulata by PCR in blood samples obtained from buffaloes supposed to be infected. The assay employs primers specific for the gene encoding Theileria annulata 185RNA biochemical changes associated with Theileria annulata in these buffaloes particular emphasis to the oxidative stress the reduce TAC level may reflect adecrease in antioxidant capacity. Blood collected from all animals on EDTA to microscopic examination and PCR to determine type of Theileria.

Abstract:Bovine babesiosis is a blood disease. Tick