Background: Human immuno-deficiency virus (HIV) counseling affirms that people exercise the right to know their HIV status, which opens the gateway to care, treatment, and support for a person in need. HIV counseling and testing uptake among sexually active males in Ethiopia is too low. Moreover, existing studies were not done at country level and the contributing factors were not well studied. Therefore, the aim of this study is to determine the status of uptake and identify its correlates using the 2016 Ethiopia Demographic and Health Survey. Methods: Data on 12,688 participants were utilized in this study. Descriptive statistics and multilevel logistic regression were used to summarize the data and investigate the associations between predictors and HIV counseling and testing uptake. Results: The overall HIV voluntary counseling and testing uptake among sexually active men in Ethiopia was 45.69%; 95% CI [43.08%, 48.33%]. About 13% of the variation in the likelihood of being tested for HIV was due to the variation among the regions. On the other hand, age, religion, education, occupation, marital status, HIV knowledge, health insurance coverage, wealth status, risky sexual behavior, family planning discussion with health workers, owning a mobile, frequency of watching television, and listening to the radio were significantly associated with the uptake of HIV voluntary counseling and testing. Conclusion: HIV voluntary counseling and testing uptake in Ethiopia is still low and varies across the regions, which might hamper the ambitious plan of Ethiopia to end AIDS as a public health threat by 2030. Therefore, giving due consideration to scale up HIV knowledge to avoid risky sexual behavior, improving access to health insurance and media, and working on the significant modifiable sociodemographic determinants are worthy to boost HIV voluntary counselling and testing uptake, which is an integral component of the strategies to efficiently prevent and control HIV.

Background Existing evidence showed that Human Immunodeficiency Virus counselling and testing uptake among Ethiopian youths is low, and factors contributing to it are not well studied. Therefore, this study aims to assess the status of uptake and identify its determinants using the 2016 Ethiopia Demographic and Health Survey data. Method Data of 10,903 Ethiopian youths were extracted from the 2016 Ethiopian Demographic and Health Survey. The association between the response variable and the predictors was modeled by multilevel binary logistic regression, whereas adjusted odds ratio and confidence intervals were used to measure associations and their statistical significance. The variation in the uptake of counselling and testing of HIV across regions of Ethiopia was quantified by intra-class correlation. Result The current study revealed that, overall, 34.9% (95% CI: 33.5, 36.2%) Ethiopian youths were ever tested for human immunodeficiency virus. Results show that about 9% of the variation in the probability of being tested for the disease was due to the regional variations. Moreover, having moderate and comprehensive HIV knowledge, being rich, having risky sexual behaviour, having a better educational level, having professional work, being married, owning of mobile, and having access to media were positively associated with human immunodeficiency virus voluntary counselling and testing uptake. On the other hand, being male, following protestant religion, following Muslim religion, and following other religions than orthodox religion were negatively associated with the uptake of human immunodeficiency virus counselling and testing. Conclusion Voluntary human immunodeficiency virus counselling and testing uptake among Ethiopian youths is very low and varies across the regions which might hamper the ambitious plan of Ethiopia to end the disease as a public health threat by 2030. Emphasis should be given to promoting the youths’ HIV-related knowledge through community-based education, encouraging and empowering the youths to participate in professional works by giving due focus to poor youths, and promoting mass media utilization to better achieve the plan.

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Background The use of synthetic insecticides against mosquitoes may lead to resistance development and potential health hazards in humans and the environment. Consequently, a paradigm needs to shift towards the alternative use of botanical insecticides that could strengthen an insecticide resistance management programme. This study aimed to assess the insecticidal effects aqueous, hexane, and methanol crude leaf extracts of Calpurnia aurea, Momordica foetida, and Zehneria scabra on an insectary colony of Anopheles stephensi larvae and adults. Methods Fresh leaves of C. aurea, M. foetida and Z. scabra were collected and dried, then separately ground to powder. Powdered leaves of test plants were extracted using sonication with aqueous, hexane, and methanol solvents. The extracts were concentrated, and a stock solution was prepared. For comparison, Temephos (Abate®) and control solutions (a mixture of water and emulsifier) were used as the positive and negative controls, respectively. Different test concentrations for the larvae and the adults were prepared and tested according to WHO (2005) and CDC (2010) guidelines to determine lethal concentration (LC) values. Mortality was observed after 24 h exposure. The statistical analyses were performed using Statistical Package for the Social Sciences (SPSS) software (Kruskal-Wallis test) and R software (a generalized linear model was used to determine LC50 and LC90 values of the extracts). Results The lowest LC50 values were observed in aqueous extracts of M. foetida followed by Z. scabra extract and C. aurea leaves at 34.61, 35.85, and 38.69 ppm, respectively, against the larvae. Larval mortality was not observed from the hexane extracts and negative control, while the standard larvicide (temephos) achieved 100% mortality. Further, the adulticidal efficacy was greatest for aqueous extract of Z. scabra with LC50 = 176.20 ppm followed by aqueous extract of C. aurea (LC50 = 297.75 ppm). Conclusion The results suggest that the leaf extracts of the three test plants have the potential of being used for the control of vector An. stephensi larvae and adult instead of synthetic mosquitocides. Further studies need to be conducted to identify the active ingredients and their mode of action.

Background Hangadi watershed is endowed with the only virgin forest in Odo shakisso harbouring high biodiversity, but it has been suffered from anthropogenic activities. This study was conducted to investigate composition and community diversity of woody species in restoration for posterity. Satellite images of 1988, 2008, and 2018 were used to classify and analyse trends of deforestation. For both woody species and topsoil (0–30 cm), 20 m × 20 m, 100 plots laid at every 300 m along line transects, 1 km apart from each other. In each sample plot, woody species ≥ 3 m were counted, Shannon–wiener diversity index, cluster analysis and ordination were computed. Results Agroforestry is found to be the dominant land use/land cover class followed by forest and cultivated land. A total of 61 woody species belonging to 34 families; 8.2% of the species were endemic to Ethiopia. The highest number of species was recorded from families Euphorbiaceae and Rubiaceae (5 species, 8.2%), Rutaceae, Celastraceae, and Oleaceae (3 species, 5.08%) followed by Flacourtiaceae, Meliaceae, Araliaceaae, Myrsinaceae, Moraceae, Boraginaceae, Asteraceae, Spontaceae, Lauraceae and Sapindaceae (2 species each). Four woody plant communities were identified using free statistical software R version 3.1.1. The canonical correspondence analysis result showed that EC, pH, OM, altitude, C:N, CEC, sand, silt, AvP, and TN significantly affected species distribution in the watershed. Conclusion Local people involved in cutting mature woody species for timber production, making farm implements and, cultivated land expansion. Protection of woody species diversity of forest and coffee systems with low biodiversity value conservation concepts are recommended to be executed jointly by local people and stakeholders.

The population status and biomass of earthworms were studied in three different land use systems of pasture (Pa), silvopasture (SP), and mixed evergreen forest (MEF) from 2019–2020 in the Solan district of Himachal Pradesh, India. The aim of this study was to assess the population status of earthworms and investigate how different land use systems influence their abundance, diversity, and biomass. Earthworms and soil were sampled using the Tropical Soil Biology and Fertility (TSBF) method in all seasons (winter, spring, summer, monsoon, and autumn). The physicochemical properties of the soil were analyzed to evaluate their effects on the diversity, biomass, and density of animals. The diversity status parameters, such as the Shannon diversity index (H′), Margalef richness index (R), evenness (J′), and dominance index (D), were computed. A total of seven earthworm species, belonging to four families, namely, Amynthas corticis, Aporrectodea rosea, Drawida japonica, Eisenia fetida, Metaphire birmanica, Metaphire houlleti, and Lennogaster pusillus, were identified from all three land use systems. The lowest Shannon diversity index (H′), Margalef index (R), and evenness (J′) index values were registered in MEF (H′ = 0.661, R = 0.762, J′ = 0.369) compared to those in Pa (H′ = 1.25, R = 1.165, J′ = 0.696) and SP (H′ = 0.99, R = 0.883, J′ = 0.552), implying that MEF is the least diversified land system. In contrast, the highest dominance index (D) value was registered in MEF (Pa = 0.39, SP = 0.53, MEF = 0.67), which again showed that MEF is the least diversified land system. The highest values of abundance and biomass were recorded in MEF (754.15 individuals m−2 and 156.02 g m−2), followed by SP (306.13 individuals m−2 and 124.84 g m−2) and Pa (77.87 individuals m−2 and 31.82 g m−2). Both the density and biomass of earthworms increased from Pa to MEF (Pa < SP < MEF). This study is novel because it revealed that the diversity and productivity (biomass and abundance) values of earthworms were negatively correlated (as diversity increased, productivity decreased; as diversity decreased, productivity increased). The total values of abundance and biomass of earthworms in the three land use systems indicated perfect synchrony between aboveground and belowground habitats, whereas the diversity values revealed that MEF was dominated by one or two species and the least diversified. Therefore, for sustainable belowground productivity, aboveground conservation is recommended, and vice versa, regardless of diversity.

Background: The use of synthetic insecticides against mosquitoes may lead to resistance development and potential health hazards in humans and the environment. In connection to these adverse effects, a paradigm needs to shift towards the alternative use of botanical insecticides that could strengthen an insecticide resistance management program. This study aimed to assess the insecticidal effects of crude leaf solvent extracts of Calpurnia aurea, Momordica foetida, and Zehneria scabra on an insectary colony of Anopheles stephensi larvae and adults. Methods: Fresh leaves were collected and dried, then separately ground to powder. Powdered leaves of test plants were extracted using sonication with aqueous, hexane, and methanol solvents. The extracts were concentrated, and a stock solution was prepared. For comparison, temephos (Synthetic larvicide) and control solutions (a mixture of water and emulsifier) were used as the positive and negative controls, respectively. A range of test concentrations for the larvae and the adults were prepared and tested according to WHO (2005) and CDC (2010) guidelines to determine lethal concentration (LC) values. Mortality was observed after 24 hrs exposure. The statistical analyses were performed using Statistical Package for the Social Sciences (SPSS) software (Kruskal-Wallis test) and R software (a generalized linear model was used to determine LC50 and LC90 values of the extracts). Results: The lowest LC50 values were observed in aqueous M. foetida extract, followed by Z. scabra and C. aurea at 34.61, 35.85, and 38.69 ppm, respectively, against the larvae. Larval mortality was not observed from the hexane and negative control, while the standard larvicide (temephos) achieved 100% mortality. In addition, the adulticidal efficacy was greatest for aqueous Z. scabra extract with LC50 = 176.20 ppm followed by aqueous C. aurea (LC50 = 297.75 ppm). Conclusions: The results suggest that the leaf extracts of the three test plants have the potential of being used for the control of vector An. stephensi larvae and adult instead of synthetic mosquitocides. Further studies need to be conducted to identify the active ingredients and their mode of action.

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