Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.

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Pulmonary edema following the relief of acute upper airway obstruction is unusual and unpredictable. The cause is usually attributed to pulmonary and hemodynamic changes resulting from high negative intrathoracic pressures during obstructed respiration. The incidence of this problem is not known. Why pulmonary edema develops in only certain patients is also unclear. We reviewed the records of 27 consecutive adult patients admitted and treated for acute upper airway obstruction. Three of 27 (11%) developed pulmonary edema. Age, sex, diagnosis, duration of obstruction, history of cardiopulmonary disease, and perioperative fluid administration were evaluated. No differences were noted between those who did and those who did not develop pulmonary edema. Pulmonary edema following acute upper airway obstruction appears to be more common than is generally appreciated, however, no specific factors seem related to its occurrence.

Summary. The yG-globulin eluted at acid pH from kidney cortex homogenates and isolated glomeruli of five of six patients with lupus nephritis was found to exhibit antinuclear activity, which was not dependent on presence of fresh human serum. Specificity, as demonstrated by absorption of antinuclear activity, was related to nucleoprotein in three glomerular acid eluates and to DNA in two acid eluates as well as in a deoxyribonuclease digest of disrupted glomeruli in one patient. Antinuclear activity was not found in acid eluates of kidneys from two patients with chronic liver disease and chronic discoid lupus, respectively, and one with lupus nephritis. These patients had a low titer of serum antinuclear factor and lesser amounts of kidney bound immunoglobulins. The presence of antinuclear activity in eluates of kidneys appeared to correlate with the amount of glomerular bound immunoglobulin and the level of antinuclear antibodies in serum. These findings suggest that in lupus nephritis, part of the glomerular bound immunoglobulin is derived from serum antinuclear factors possibly deposited as immune complexes. IntroductionThe definition of immunoglobulin deposits in lupus kidneys has been approached by techniques applicable to dissociation of antigen-antibody complexes. Sections of lupus nephritis kidneys washed at acid pH (3.0 to 3.2) were found to lose considerable staining for y-globulin in glomerular and vas-

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Radiolabeled microspheres were used to measure gingival and alveolar bone blood flow in beagles with periodontal disease. The severity of periodontal disease was determined using radiographs, and the percent alveolar bone loss around specific tooth areas was calculated. Gingiva and alveolar bone blood flows were lowest in dogs with minimal periodontal disease (< 10% bone loss) while dogs with moderate to severe disease (> 20% bone loss) had periodontal blood flows 250–400% greater (p < .05). In all animals maxillary alveolar bone had higher blood flow than corresponding mandibular bone (p < .05) whereas gingival blood flow between jaws was similar.