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Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.

This paper and those which accompany it describe a method for the specific histochemical demonstration of antibody, and present data gleaned by its use concerning the response to antigenic stimulation. These data indicate that the major site of antibody formation is a family of ceils which first appear as a response to the stimulus. The response consists of cell multiplication, cell differentiation, and the concurrent synthesis of a specific protein, antibody. The mature member of this cell family is the plasma cell.

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