Objectives The aim of this study was to explore the Vitamin D receptor (VDR) gene polymorphism and its association with multiple myeloma (MM) development. Methods The peripheral blood of 40 MM cases and 84 healthy controls were collected. Polymerase chain reaction (PCR) and DNA sequencing were applied to detect VDR gene polymorphism (including: FokI, BsmI, ApaI, and TaqI). SHESIS biological information software was used to analyze genotypes, alleles, linkage disequilibrium (LD), haplotype distribution, and their association with MM. Results Compared with controls, the MM group had a significantly higher frequency of the A allele in BsmI site (8.7% vs 2.4%) and C allele in the TaqI site (10.5% vs 3.6%). These two alleles were closely associated with an increased risk of MM (P = .025; P = .030). The highly rare genotypes (BsmI‐AA and TaqI‐CC) were found in one patient with MM. Conclusion VDR gene polymorphisms may be a molecular marker of MM risk.

Objective: By comparing the results of moxifloxacin sensitivity test and clinical efficacy in nontuberculous mycobacterium lung disease (NTM-PD) further confirm the necessity of moxifloxacin as a first-line drug for the treatment of nontuberculous mycobacterium（NTM）.Method: Positive samples of sputum, bronchoscope brush or alveolar lavage fluid cultured in Bactec MGIT960 system were retrospectively selected from inpatients in Shandong Provincial Chest Hospital, China from January 2015 to December 2020. The strains were identified by MPB64 colloidal gold method, gene chip and microbial mass spectrum identification system. The drug detection of NTM was performed by microplate method, Meanwhile, the efficacy of moxifloxacin in NTM-PD treatment regimen was compared.Results: 1. Among the 575 patients with positive sample culture, the bacteria species of 485 patients were identified. A total of 19 species of NTM were detected, and the majority of them were Mycobacterium intracellulare (68.87%, 334/485).2.The results of microplate assay of 219 NTM patients showed that all NTM strains were highly sensitive to moxifloxacin, amikacin and clarithromycin [100.00%(219/219), 96.80%(212/219), 94.52% (207/219), respectively]. 3. This study showed that the bacteriological cure rate of the NTM-PD treatment regimen containing moxifloxacin was 73.68% (42/57), significantly higher than that of the regimen without moxifloxacin (55.45%, 56/101), and the difference was statistically significant (P<0.05).Conclusion: In this study, all the strains of NTM patients were 100% sensitive to moxifloxacin, and the clinical bacteriological cure rate of moxifloxacin containing regimen was significantly improved. Therefore, moxifloxacin is recommended as the first-line drug for the treatment of NTM.