Abstrak Patikala (Etlingera elatior (Jack) R.M.Sm) adalah tanaman dari keluarga Zingiberaceae. Patikala memiliki senyawa bioaktif adalah flavonoid yang berfungsi sebagai antioksidan. Tujuan dari penelitian ini adalah untuk mengukur aktivitas radikal bebas dalam ekstrak metanol bunga dan daun Patikala. Ekstraksi bunga dan daun sampel dengan metode maserasi menggunakan metanol. Analisis kualitatif senyawa kimia dengan eluen tertentu dengan menggunakan Kromatografi Lapis Tipis (KLT) untuk menentukan kelompok senyawa aktif dalam ekstrak. Uji aktivitas antioksidan menggunakan metode perendaman DPPH (1,1-Diphenyl-2-picryl Hidrazil) diukur serapan pada panjang gelombang 517 nm dan dibandingkan dengan kekuatan quersetin antioksidan. Hasil penelitian menunjukkan bahwa ekstrak metanol di 1,935% untuk bunga dan daun pada 5.17%. Aktivitas antioksidan ekstrak metanol bunga patikala memiliki aktivitas antioksidan yang rendah dengan nilai IC 50 101,84 mg / mL dan metanol ekstrak daun Patikala memiliki aktivitas antioksidan yang kuat dengan nilai IC 50 30,65 mg / mL. Potensi ini lebih rendah dari nilai IC 50 quercetin dari 5,35 mg / mL. Abstract Patikala (Etlingera elatior (Jack) R.M.Sm) is a plant of the Zingiberaceae family. Patikala (E. elatior (Jack) R.M.Sm) have bioactive compounds are flavonoids that act as antioxidants. The purpose of this study was to measure free radical activity in the methanol extract of the fruit and leaves Patikala (E. elatior (Jack) R.M.Sm). Extraction of flowers and leaf samples by maceration method using methanol. Qualitative analysis of chemical compounds with certain eluent using Thin Layer Chromatography (TLC) to determine the group of active compounds in the extract. Antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 517 nm and compared with the power of antioxidant quersetin. The results showed that the methanol extract rendemen is at 1.935% for flowers and leaves at 5.17%. Antioxidant activity of the methanol extract of the flowers patikala (E. elatior (Jack) R.M.Sm) have low antioxidant activity with the value IC 50 101.84 mg / mL and methanol extracts of leaves Patikala (E. elatior (Jack) R.MSm) have strong antioxidant activity with value IC 50 30,65 mg / mL. This potential is lower than that of quercetin IC 50 value of 5.35 mg / mL.
Schistosomiasis di Indonesia hanya ditemukan di tiga lokasi yaitu Dataran Tinggi Napu dan Dataran Tinggi Bada, Kabupaten Poso serta Dataran Tinggi Lindu, Kabupaten Sigi Propinsi Sulawesi Tengah. Hasil survei fokus keong ada fokus keong yang berada di kawasan Taman Nasional Lore Lindu (TNLL) Sehingga banyak masyarakat menduga bahwa penyebaran fokus keong O.hupensis lindoensis juga terdapat di TNLL. Tujuan untuk memetakan daerah fokus keong perantara schistosomiasis di kawasan Taman Nasional Lore Lindu. Lokasi penelitian di 12 Desa yang masuk kawasan TNLL di Dataran Tinggi Napu, Dataran Tinggi Bada Kab. Poso dan Dataran Tinggi Lindu Kab. Sigi. Melakukan survei fokus /habitat dan survei keong. Pada kedua kegiatan ini dilakukan penentuan koordinat geografis/UTM dengan menggunakan GPS.Hasil survei menunjukan bahwa ditemukan 14 daerah fokus keong O.hupensis lindoensis yang tersebar di tiga desa yaitu Desa Sedoa, Desa Dodolo Kec.Lore Utara Kab. Poso dan Desa Anca Kec. Lindu Kab. Sigi
Abstract Gerakan 1 Rumah 1 Jumantik (G1R1J) is an effective effort to prevent the DBD in Poso District. However, the DBD case remains to fluctuate annually. The purpose of the research is to assess the community's knowledge, attitudes, and actions before Kawua Village intervention and after the intervention on G1R1J as well as comparing with the Sayo Village as a control area. Research design using the quasi-experimental with a control method. Data Collection is done to the public to know the knowledge, attitudes, and behaviors of The G1R1J program in the intervention area and compare it to the control region. The total sample of 150 households for each part is the intervention and control region. Statistical analysis using the dependent T-test. There are differences in the average knowledge, attitudes, and actions of respondents before and after intervention in the Kawua region (intervention area). There is a significant difference in knowledge, attitudes, and acts of respondents after intervention in the Kawua region (intervention area) and Sayo region (non-intervention area). There was a change in the rate of knowledge, attitudes, and actions of respondents after the intervention of socialization and mentoring four times. Abstrak Gerakan 1 Rumah 1 Jumantik (G1R1J) merupakan upaya yang efektif untuk mencegah penularan DBD di Kabupaten Poso. Namun, kasus DBD tetap berfluktuasi setiap tahunnya. Tujuan penelitian adalah untuk menilai pengetahuan, sikap dan tindakan masyarakat Kelurahan Kawua sebelum diberikan intervensi dan setelah intervensi tentang G1R1J serta membandingkan dengan Kelurahan Sayo sebagai wilayah yang tidak dilakukan intervensi (kontrol). Disain penelitian menggunakan metode quasi experimental with control. Pengumpulan data dilakukan kepada masyarakat untuk mengetahui pengetahuan, sikap dan perilaku terhadap program G1R1J di wilayah intervensi serta membandingkannya dengan wilayah kontrol. Jumlah sampel sebanyak 150 rumah untuk masing-masing wilayah yaitu wilayah intervensi dan wilayah kontrol. Analisis statistik menggunakan uji T dependen. Terdapat perbedaan rerata pengetahuan, sikap dan tindakan responden yang signifikan sebelum dan setelah intervensi di wilayah Kelurahan Kawua (wilayah intervensi). Terdapat perbedaan rerata pengetahuan dan sikap responden yang signifikan setelah intervensi di wilayah Kelurahan Sayo (non intervensi). Ada perubahan pengetahuan, sikap dan tindakan responden setelah diberikan intervensi sosialisasi dan pendampingan empat kali menjadi lebih baik.
Abstract Leptospirosis is an endemic zoonotic disease and remains a health problem in Indonesia. The word’s third-heighest cases of leptospirosis. This study aimed to determine the type of mouse as an intermediate transmission of leptospirosis disease in Minahasa District, North Sulawesi. Trapping of rats was performed by using 100 mouse traps (live Trap) for two consecutive days across six ecosystems. Rats were identified by external morphological characteristics. Microscopic Agglutination Test (MAT) and Polymerase Chain Reaction (PCR) were conducted using rat kidney samples. The number of rats caught in the present study was 105 heads consisting of six species e.g Rattus tanezumi, Rattus exulans, Rattus hoffmanni, Bunomis coelestis, Bunomis fratorum, and Paruromys dominator. The proportion of rats identified as MAT-based leptospirosis reservoir was 0.9% while the PCR test was 1.9%. Rattus tanezumi identified as leptospirosis reservoirs were found were found in the NHDP (non forest near to sattlement) ecosystem. Rattus tanezumi trapped near the settlement was positive as leptospirosis reservoirs in the Minahasa District. Abstrak Leptospirosis merupakan penyakit zoonosis endemik dan masih menjadi masalah Kesehatan di Indonesia. Angka CFR penyakit ini dilaporkan sebesar 2,5 – 16,4% atau rata-rata 7,1% sehingga menempatkan Indonesia sebagai negara peringkat ketiga tertinggi di dunia. Penelitian ini bertujuan untuk mengetahui jenis tikus perantara penyakit leptospirosis di Kabupaten Minahasa Provinsi Sulawesi Utara. Penangkapan tikus dilakukan dengan menggunakan 100 perangkap tikus (live trap) selama 2 hari berturut-turut di enam ekosistem. Tikus diidentifikasi dengan melihat karakteristik morfologi eksternalnya kemudian dilakukan pemeriksaan secara biomolekuler yaitu uji Microscopic Aglutination Test (MAT) dan Polimerase Chain Reaction (PCR) terhadap sampel ginjal tikus. Jumlah tikus yang tertangkap sebanyak 105 ekor yang terbagi menjadi enam spesies yaitu: Rattus tanezumi, Rattus Exulans, Rattus hoffmanni, Bunomis coelestis, Bunomis fratorum, dan Paruromys dominator. Proporsi tikus yang teridentifikasi sebagai reservoir leptospirosis berdasarkan uji MAT adalah 0,9% dan sebesar 1,9% berdasarkan uji PCR. Spesies Rattus tanezumi teridentifikasi sebagai reservoir leptospirosis dan ditemukan di ekosistem Non Hutan Dekat Pemukiman (NHDP). Beberapa Rattus tanezumi yang tertangkap di lokasi dekat pemukiman teridentifikasi positif sebagai reservoir leptospirosis di Kabupaten Minahasa.
The provision of knowledge on schistosomiasis to the students and teachers of elementary schools increase their knowledge and affected the coverage of stool collection. Teachers and students are also expected to be able to provide information about the schistosomiasis which they have obtained to their families and communities around them. This research aimed to assess the success of the training of teachers and students in the control of schistosomiasis. This research was intervention research that used quasi-experiment design. The intervention was conducted by providing socialization about schistosmiasis to students and teachers in classrooms, followed by the practice in the field. The intervention and control group comprised 63 students and teachers. Evaluation of the teachers and students knowledge about schistosomiasis was conducted by questionnaire. The results of this research showed that in the intervention group, there was a significant increase in the knowledge from pre-test 9,25 to post-test 16,86 (p = 0.000). The provision of intervention in the form of knowledge to students and teacher has significantly increased the knowledge about epidemiology, treatment and surveillance schistosomiasis.
Abstract Malaria remained a public health problem at the global level, as well as in Indonesia. In Banggai Islands District and Tojo Una-una District, the API (Annual Parasite Incidence) tends to decrease every year. Village institutions and local community leaders are quite influential in reducing malaria cases. This study aimed to explore the policy reinforcing factors in malaria control, including malaria program policies and the involvement of local institutions in controlling malaria. The type of this study was qualitative with the Rapid Assessment Procedure. Data collection techniques with triangulation through in-depth interviews, Focus Group Discussion, observation, and study of documents. Analysis data use domain analysis. The samples were selected through snowball sampling, consists of community leaders, malaria policy program makers at the district, health center and community who had been diagnosed with malaria. Retrieval of data with triangulation techniques to compared analysis. The results of the study showed that the collaboration between the Banggai Islands and Tojo Una-una District Health Office and the relevant agencies, in resolving malaria elimination as stipulated in the regent's regulations, the stakeholders are still carrying out their respective programs. The policy strengthening factor in malaria control in Tojo Una-una District is the existence of a malaria-free declaration from 2015 by local institutions and local communities, as well as local wisdom of the Banggai Islands District in the form of village race activities as an indicator of public health and environmental sanitation. Abstrak Malaria masih merupakan masalah kesehatan masyarakat di tingkat global, demikian pula di Indonesia. Kabupaten Banggai Kepulauan dan Kabupaten Tojo Una-una memiliki API (Annual Parasite Incidence) cenderung menurun setiap tahunnya. Lembaga desa dan tokoh masyarakat memegang peran penting dalam penurunan kasus malaria. Tujuan penelitian adalah mengeksplorasi faktor penguat kebijakan dalam pengendalian malaria yaitu, kebijakan program malaria dan keterlibatan institusi lokal dalam pengendalian malaria. Jenis penelitian ini adalah kualitatif dengan rancangan Rapid Assessment Procedure. Teknik pengumpulan data dengan triangulasi melalui wawancara mendalam, Focus Group Discussion, observasi dan telaah dokumen. Analisis data menggunakan analisis domain. Pemilihan sampel secara snowball sampling, sampel terdiri dari tokoh masyarakat, penentu kebijakan program malaria di tingkat kabupaten, puskesmas dan masyarakat yang pernah positif malaria. Hasil penelitian menunjukkan kolaborasi antara Dinas Kesehatan Kabupaten Banggai Kepulauan dan Kabupaten Tojo Una-una dengan instansi terkait, dalam pencapaian eliminasi malaria yang tertuang dalam peraturan bupati belum berjalan dengan baik, stakeholder masih menjalankan programnya masing-masing. Faktor penguat kebijakan dalam pengendalian malaria di Kabupaten Tojo Una-una adalah adanya deklarasi bebas malaria tahun 2015 oleh institusi lokal dan masyarakat setempat, serta kearifan lokal pemerintah Kabupaten Banggai Kepulauan berupa kegiatan lomba desa sebagai indikator kesehatan masyarakat dan sanitasi lingkungan.
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