Desa Beji salah satu desa di Kota Batu yang terkenal dengan pengrajin tempe. Desa ini menjadi kampung terbesar tempe kedua di Malang raya setelah Sanan. Tempe merupakan salah satu warisan budaya tak benda yang diajukan ke UNESCO. Keunggulan ini kurang ditonjolkan sehingga perlu dilakukan pendampingan serta pengembangan di desa Beji yang potensinya di kota Batu sangat menjanjikan. Batu yang kerap dengan kota wisata menjadi pusat para wisatawan baik domestic maupun mancanegara. Sehingga dengan menggali potensi di Beji maka dapat mengenalkan bagaimana desa ini berkembang menjadi pusat wisata edukasi tempe. Metode pengabdian dilakukan melalui transfer keilmuan ke masyarakat dan pengrajin tempe, khususnya kelompok wanita Tani Asri, dikemas dalam penyampaian materi secara klasikal, workshop dan praktik pengenalan wisata edukasi tempe, pendampingan entrepreneur dan evaluasi. Kegiatan pengabdian masyarakat ini memiliki beberapa tahapan dimulai dari identifikasi permasalahan mitra perlunya edukasi pentingnya wisata tempe bagi Desa dan pembekalan transfer keilmuan tentang tempe. Hal ini terlihat dari hasil survei terhadap pentingnya wisata edukasi di desa ini yang menunjukkan 90%, pendampingan dari hasil entrepreneur 85%, promo diadakanya Festival Beji Kampung Tempe 97%, sehingga didapatkan hasil kepuasan keseluruhan sebesar 87%.
Microfluidic paper-based analytical device has been fabricated and subsequently applied to determine the Vitamin C level of the non-seasonal fruit i.e pineapples, oranges, guavas, and apples in Indonesia. µPAD was prepared by creating a hydrophobic barrier on Whatman Chromatography paper No. 1 in a certain pattern printed using Xerox Colorcube 8580 DN-2. 0.05 µl of 2,6-Dichlorophenolindophenol was dispersed into a detection area on the µPAD devices prior to the addition of 0.2 µl of fruits juice samples. The barrier allowed the fruits juice to flow and alter the color of the reagents through a redox reaction. Vitamin C in fruit juice altered the color of 2.6-Dichlorophenolindophenol reagent into a pinkish color. The changing of the reagent color was then processed using Image J software to determine the RGB intensity level. The results showed that the average amounts of Vitamin C in pineapples, oranges, guavas, and apples are 0.05%, 0.05%, 0.08%, and 0.04% respectively.
The determination of the antioxidant activity in green tea using FRAP essays developed in a Microfluidic Paper-Based Analytical Device (μPADs) was observed. μPAD was prepared on the chromatographic paper with a suitable pattern and then printed using a solid ink printer. The solid printing was intended to obtain a hydrophobic barrier and a hydrophilic channel on the chromatographic paper. A preliminary study was done to determine the optimum time and temperature used on the penetration of obtaining the hydrophobic barrier to avoid leakage in the channel. Optimization of temperature and time was calculated by the average velocity, the optimum condition was obtained at 120 °C for 90 seconds with an average speed of 0.1 mm.s−1. The green tea samples were prepared by extracting its active compound using demineralized water at 25 °C and 90 °C with 2 hours of immersion time. For the measurement of antioxidant activities, the analysis was carried out by placing 0.5 μl in the detection zone and 5 μl samples into the sample zone μPAD. Then, the color reaction product, which propagates from the sample zone to the detection zone, is processed by Image J software to measure color intensity in CMYK mode. Extracts of green tea samples at 25 °C and 90 °C has a significant difference in antioxidant activity. These results indicate that the method developed in this work can be used as an alternative method for analyzing antioxidant activity in green tea extract. The results of the average amount of antioxidant activity in green tea samples were shown with Fe2+ concentration. High-speed detection, low cost, high accuracy, and ease of use can be attributed to the advantages of our μPAD method.
Kepok banana corm is one of the natural ingredients that has been obtained and can provide many benefits because of its excessive flavonoid content. This study aims to determine the types of flavonoids contained in the kepok banana weevil extract. Methods of detection and discovery of flavonoid measurement methods used include reaction, thin layer chromatography, and LC-MS/MS, and spectrofotometri for total flavonoid content compared to rutin as a flavonoid standard. The results showed that kepok banana corm extract contains a class of flavonoid compounds including rutin, kaempferol 3-O-rhamnoside-7-O-glucoside, quercetin 3 glycocide, and chrysoeriol-7-O-glycuronyl, with a total flavonoid content of 6.19. ±0.65%.
Pisang dapat ditemukan di semua di daerah tropis di Indonesia dan memiliki berbagai jenis. Tidak hanya buahnya tapi bonggol pisang juga memiliki kandungan yang bermanfaat yaitu pektin. Pektin merupakan golongan polisakarida pada dinding sel tumbuhan yang mengandung asam galakturonat yang salah satu fungsinya adalah sebagai pengawet. Penelitian ini bertujuan untuk mengekstraksi pektin dari bonggol pisang dan kemudian dilakukan karakterisasi untuk menentukan mutu pektin. Penelitian ini dilakukan dengan metode ekstraksi menggunakan pelarut HCl kemudian ditambahkan etanol ke dalam filtrat untuk mengendapkan pektin dan kemudian dilakukan pengeringan untuk mengendapkan pektin kering. pektin kering kemudian dilakukan karakterisasi dengan beberapa parameter, diantaranya rendemen, bobot pektin, kadar air, kadar abu, berat ekivalen, kadar metoksil, kadar asam galakturonat, dan derajat esterifikasi. Hasil dari pengujian didapatkan data rendemen sebesar 19,6546%; kadar air sebesar 8,19%; kadar abu sebesar 0,3494%; berat ekivalen sebesar 625,211; kadar metoksil sebesar 3,27%; kadar asam galakturonat sebesar 321,94%; dan derajat esterifikasi sebesar 16,89%.
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