Effects of different levels of Biomin® IMBO synbiotic, including Enterococcus faecium (as probiotic), and fructooligosaccharides (as prebiotic) on survival, growth performance, and digestive enzyme activities of common carp fingerlings (Cyprinus carpio) were evaluated. The experiment was carried out in four treatments (each with 3 replicates), including T1 = control with non-synbiotic diet, T2 = 0.5 g/kg synbiotic diet, T3 = 1 g/kg synbiotic diet, and T4 = 1.5 g/kg synbiotic diet. In total 300 fish with an average weight of 10 ± 1 g were distributed in 12 tanks (25 animals per 300 l) and were fed experimental diets over a period of 60 days. The results showed that synbiotic could significantly enhance growth parameters (weight gain, length gain, specific growth rate, percentage weight gain) (P < 0.05), but did not exhibit any effect on survival rate (P > 0.05) compared with the control. An assay of the digestive enzyme activities demonstrated that the trypsin and chymotrypsin activities of synbiotic groups were considerably increased than those in the control (P < 0.05), but there was no significant difference in the levels of α-amylase, lipase, or alkaline phosphatase (P > 0.05). This study indicated that different levels of synbiotic have the capability to enhance probiotic substitution, to improve digestive enzyme activity which leads to digestive system efficiency, and finally to increase growth. It seems that the studied synbiotic could serve as a good diet supplement for common carp cultures.

In this study, the effects of olive leaf extract on growth, haematological parameters, immune system and carcass composition in common carp were examined. In this research, 180 common carp (32.26 ± 1.01 g) were randomly distributed to nine fibreglass tanks. The fish were fed a diet with 200 mg/kg olive leaf extract (group 1), 400 mg/kg olive leaf extract (group 2), and basal food (control group) for 75 days. At the end of the experiment, WG, SGR and PER values were significantly higher in the groups fed the diet containing 200 mg/kg of olive leaf extract compared with the control group. Furthermore, FCR and FER values were improved in the groups fed by diets with various levels of olive leaf extract than the control group. The contents of WBCs, lysozyme and NBT activity were significantly increased in the groups fed diets containing olive leaf extract. Cholesterol content was significantly lower in the group fed diet containing olive leaf extract compared with the control group. Also, serum triglyceride and carcass lipid contents were significantly lower in the group fed diet containing 200 mg/kg of olive leaf extract compared with the control group. Total protein, albumin, globulin and calcium contents were significantly higher in the group fed the diet containing 200 mg/kg of olive leaf extract compared with the control group. Our results demonstrated that diet containing 200 mg/kg olive leaf extract were efficient feed additives and has the potential for use in improving the growth performance and health status for this species.

This research was conducted to evaluate qualitative changes of Benni Fish (Barbus Sharpeyi) during its maintenance in ice storage for 20 days. To do so, chemical spoilage indicators including peroxide (PV), thiobarbituric acid (TBA), free fatty acids(FFA), total lipid (TL), moisture (M), heme iron (HI), and also organoleptic parameters (tissue, gill appearance, gill smell, general appearance, and eyes) were measured. Fat quality of sample fish (in terms of oxidative and hydrolytic rancidity) showed a significant reduction during the maintenance period (p<0.05). Peroxide index changes from 3.73 to 7.52 (meq/kg) and TBA from 0.5 to 6.6 (mg MDA/kg) was recorded as markers of oxidative spoilage and FFA changes from 2.05 to 6.58 (expressed as % of oleic acid) were recorded as indicator of hydrolytic rancidity. Each one of sensory tests were rated as excellent to good until the fourth day and their quality was acceptable until the tenth day and then organoleptic results dropped significantly. In general, the best time of fish Shelf life in ice storage was determined to be 7 to 10 days.

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The present study was conducted to determine the optimum weaning time of shirbot, Barbus grypus larvae with formulated diet.

In recent years, the aquaculture industry has extended significantly and has moved towards intensive and super-intensive systems due to the growth in the world population and the demand to provide food from diverse sources. As a result, the importance of the need for water quality control, nutrition and disease prevention is increasingly felt in the aquaculture industry (Chelemal Dezfoulnejad & Molayemraftar, 2021;FAO, 2021). In the past decades, many researchers have studied various aspects of the administration of

Background: Sea foods contain high level of unsaturated fatty acids. The aim of this study is comparison of ώ 3 and ώ 6 levels in wild and farmed green tiger shrimp (P.semisulcatus). Materials and Methods: in this study 120 shrimps (wild=60 and farmed=60) selected. Analysis of Fatty acids was performed by Gas Chromatography. Two-factor variance difference analysis used for demonstration the effects of main factor (sex and environment) and their interactions. Results: Three fatty acids included linoleic, Decosahexaenoic (DHA) and eicosapentaenoic (EPA) were the most dominant fatty acids in the wild and farmed species. Among ώ 6 fatty acids, the farmed shrimp contained a higher level of linoleic acids (P>0.05) whereas in ώ 3 fatty acids, eicosapentaenoic acid (EPA) and eicosathereanoic acid were significantly higher in wild shrimps. The n-3/n-6 fatty acids ratio was similar in two groups. The comparison of DHA/EPA ratio did not show difference between wild and farmed shrimps and this ratio was affected by sex. Conclusion: Comparison between two groups showed that the concentration of fatty acids is relatively to each other in wild and farmed shrimp.