DNA barcoding has been proposed as a means of quick species identification using a short standardized segment of DNA. Two species (Eleotris fusca and Glossogobius giuris) from the family Gobiidae and Eleotridae were selected for DNA barcoding using samples collected from different regions of Bangladesh. Cytochrome Oxidase Subunit I (COI) gene was sequenced from two different gobi fishes and compared with two previously published similar sequences from the genera Eleotris and Glossogobius. Multiple sequence alignment and the molecular systematic study were performed. The DNA barcode technique identified the two species. The study provides a good example of how DNA barcoding can build upon its primary mission of species identification and use available data to integrate genetic variation investigated at the local scale into a global framework.
Work on two freshwater Gobi fish species such as Awaous guamensis and A. grammepomus was carried out on morphometric and meristic study for their morphological identification. Sequence data of mitochondrial cytochrome oxidase subunit I (COI) gene was used for this purpose. Around 620 bp sequences were obtained when universal primers were used. An alignment was performed between the sequences which showed some inter-specific variation. An entropy plot was generated for all the aligned positions which showed that the entropy rarely touches a scale of one, which is a sign of better alignment in the region. This work can be utilized for species identification, phylogenetic analysis, to study molecular diversity and evolution, and most importantly for the conservation of these fauna in near future.
Dhaka Univ. J. Biol. Sci. 26(1): 83-90, 2017 (January)
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