Identification of Awaous guamensis and A. grammepomus based on morphology and molecular analysis

Abstract

Work on two freshwater Gobi fish species such as Awaous guamensis and A. grammepomus was carried out on morphometric and meristic study for their morphological identification. Sequence data of mitochondrial cytochrome oxidase subunit I (COI) gene was used for this purpose. Around 620 bp sequences were obtained when universal primers were used. An alignment was performed between the sequences which showed some inter-specific variation. An entropy plot was generated for all the aligned positions which showed that the entropy rarely touches a scale of one, which is a sign of better alignment in the region. This work can be utilized for species identification, phylogenetic analysis, to study molecular diversity and evolution, and most importantly for the conservation of these fauna in near future.
 Dhaka Univ. J. Biol. Sci. 26(1): 83-90, 2017 (January)

Morphological characteristic and truss morphometric analysis of amphidromous goby (‘nike') (Teleostei: Gobiiformes) in Bone River, Gorontalo, Indonesia

“…The thirteen morphological trusses which are not recommended to be used to differentiate sexuality in A. grammepomus are distance between posterior base of the dorsal fin and the anterior base of the second dorsal fin, distance between anterior base of the anal fin and the anterior base of the ventral fin, distance between outer tip of the snout and the anterior base of the ventral fin, distance between headdorsal body border point and the anterior base of the anal fin, distance between head-dorsal body border point and the anterior base of the ventral fin, distance between anterior starting point of the dorsal fin and anterior base of the ventral fin, distance between posterior starting point of dorsal fin and anterior base of the ventral fin, distance between anterior base of ventral fin and posterior base of anal fin, distance between anterior base of the second dorsal fin and anterior base of anal fin, distance between posterior base of the second dorsal fin and anterior base of ventral fin, distance between posterior base of the second dorsal fin and anterior base of anal fin, distance between anterior base of dorsal caudal fin and posterior base of anal fin, and distance between anterior base of dorsal caudal fin and midpoint of anterior base of the caudal fin. Standard length of A. grammepomus reported by Jahan et al (2017) is 82 mm. This size is longer than the fish samples in this research which only measured 53.04 to 54.84 mm.…”