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The purpose of this study was to examine the possible effect of extremely low-frequency electromagnetic fields (ELF-EMFs), from a high-voltage source, on rat teeth in terms of changes in trace elements (TEs) and the effect of antioxidants (melatonin [MLT] and Ganoderma lucidum [GL]) in counteracting these effects. We used adult male Wistar albino rats with a mean weight of 250–300 g and divided the rats into eight groups. The groups were subjected to an ELF-EMF that was applied with a high-voltage line for 8 hours/day for 26 days (Groups I, II, and III) or 52 days (Groups V, VI, and VII). Groups IV and VIII were the 26- and 52-day control/sham groups, respectively. Groups II and VI were treated with GL, and Groups III and VII were treated with MLT. MLT and GL were administered daily based on the weight of the animals and appropriate standards. At the end of the study, the rats were euthanized, and their anterior teeth were extracted. The teeth were preserved in pure water before evaluating the major TEs. At the end of the study, TE concentrations (in mg/kg) were assessed in the control and test groups. Compared with Group V, statistically significant differences in the concentrations of zinc (Zn) and strontium (Sr) were found for Group VII (ELF-EMF + MLT) (P<0.05). Therefore, ELF-EMF exposure can change the content of certain TEs in teeth and, after administering MLT and GL, the values of some of the TEs return to normal.

Background/aim: The aim of this study was to determine whether Extremely low frequency electric and magnetic field (ELF-EMF) had any harmful effects on dental pulp tissue and examine histologically whether melatonin (MLT) and Ganoderma lucidum (GL) play a protective role against these harmful effects. Material and method: A total of 56 adult, male Wistar Albino rats were used in the study. The rats were separated into 8 equal groups and were exposed to ELF-EMF, generated by high voltage source, for 8 hours/day for 26 days-Groups I, II and III and for 52 days-Groups V, VI and VII. For sham-control groups (Groups IV and VIII for 26 and 52 days), rats were placed into same experimental setup as exposure groups and same procedure was applied except ELF-EMF exposure. GL (20 mg/kg/day) and MLT (10 mg/kg/day) were administered by oral gavage and the intraperitoneal route respectively. At the end of the study, the rats anterior teeth were examined immunohistochemicaly using vimentin and vascular endothelial growth factor (VEGF). Results: Degeneration in the odontoblast cells, inflammatory cell infiltration, dilatation of the blood vessels and haemorrhagic changes were determined in the pulp of the rats in Groups I and V. A statistically significant difference was determined in the groups treated with MLT and GL (p < 0.05). Conclusion: It was determined that exposure to ELF-EMF induced histological changes in the dental pulp of rats, the using of MLT and GL could have a protective effect against these effects.

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Methods: In the study, 64 rats were divided into eight equal groups (n: 8). In experiments lasting 26 and 52 days, the following groups were used: High voltage (HV), HV + Ganoderma lucidum (GI), HV + Melatonin (MEL), and Shamcontrol. MEL (10 mg/kg) was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremely low frequency electric (80.3 V/m) and magnetic fields (2.48 µT) were applied for eight hours per day.