In acoustic-resolution photoacoustic microscopy (AR-PAM) systems, the lateral resolution in the focal zone of the ultrasound (US) transducer is determined by the numerical aperture (NA) of the transducer. To have a high lateral resolution, a large NA is used. However, the larger the NA, the smaller the depth of focus [DOF]. As a result, the lateral resolution is deteriorated at depths out of the focal region. The synthetic aperture focusing technique (SAFT) along with a beamformer can be used to improve the resolution outside the focal region. In this work, for image formation in AR-PAM, we propose the double-stage delay-multiply-and-sum (DS_DMAS) algorithm to be combined with SAFT. The proposed method is evaluated experimentally using hair targets and in vivo vasculature imaging. It is shown that DS_DMAS provides a higher resolution and contrast compared to other methods. For the B-mode images obtained using the hair phantom, the proposed method reduces the average noise level for all the depths by about 134%, 57% and 23%, compared to the original low-resolution, SAFT+DAS and SAFT+DMAS methods, respectively. All the results indicate that the proposed method can be an appropriate algorithm for image formation in AR-PAM systems. K E Y W O R D S acoustic-resolution photoacoustic microscopy, contrast enhancement, synthetic aperture focusing technique, vasculature imaging, virtual source Moein Mozaffarzadeh, Mehdi H. H. Varnosfaderan and Arunima Sharma contributed equally to this study.
In recent years, the minimum variance (MV) beamformer has been highly regarded since it provides high resolution and contrast in B-mode ultrasound imaging compared with nonadaptive delay-and-sum (DAS) beamformer. However, the performance of MV beamformer is degraded in the presence of the noise due to inaccurate estimation of the covariance matrix resulting in low-quality images. The conventional tissue harmonic imaging (THI) offers multiple advantages over conventional pulse-echo ultrasound imaging, including enhanced contrast resolution and improved axial and lateral resolutions, but low signal-to-noise ratio (SNR) is a major problem facing this imaging method, which uses a fixed transmit focus and dynamic receive focusing (DRF). In this paper, a synthetic aperture method based on the virtual source, namely, bidirectional pixel-based focusing (BiPBF), has been combined with the MV beamformer and then applied to second-harmonic ultrasound imaging. The main objective is suppressing the noise level to enhance the performance of the MV beamformer in the harmonic imaging, especially in lower and deeper depths where the SNR is low. In addition, combining the BiPBF and MV weighting results in simultaneous improvement in imaging resolution and contrast, in comparison with the conventional methods: DRF (DAS), BiPBF (DAS), and DRF (MV). The performance of the proposed method is evaluated on simulated and experimental RF data. The THI is achieved using the pulse-inversion technique. The results of the simulated wire phantom demonstrate that the proposed beamformer can achieve the best lateral resolution, along different depths, compared with DRF (DAS), BiPBF (DAS), and DRF (MV) methods. The results of the simulated and experimental cyst phantoms show that the new beamformer improves the contrast ratio (CR) and contrast-to-noise ratio (CNR) of the resulting images. In results of simulated cyst phantom, in average, the new beamformer improves the CR and CNR of the cyst about (7.4 dB, 49%), (3.2 dB, 16%), and (5 dB, 26%) compared with DRF (DAS), BiPBF (DAS), and DRF (MV), respectively. In results of experimental cyst phantom, these relative improvements are about (4.2 dB, 22%), (1.7 dB, 7%), and (2.6 dB, 15%). In addition, BiPBF (MV) method offers improved edge definition of cysts in comparison with the other methods.
Traditional optical devices rely on light propagation along a straight path. However, when the light propagates through a blurred medium, its direction get scattered by microscopic particles. This inhomogeneous distortion results in a diffused focus point. Light scattering is one of the main limitations for the optical imaging. This limitation decreases the resolution in depth. Therefore, the ability of focusing light at a desired position has a huge worthwhile for applications of optical imaging. Over the past few years, it was shown that light can be focused inside an object even with strong scattering particles, just by shaping the wavefront of the incident beam. The most successful approaches for light focusing at the presence of scattering objects are feedback-based optical wavefront shaping. In this paper, an iterative feedback-based wavefront shaping is proposed. It uses the genetic algorithm. In summary, we aim to obtain a high intensity in the focus point with fewer steps in iteration while increase the signal-to-noise. The simulations results show that both the above mentioned goals are achieved using the proposed method.
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