White clover (cv. Grasslands Huia), red clover (cv. Norseman) and lucerne (cv. Sabilt) were sampled during primary growth at the flower bud, near full flowering and seeds formed stages in each of 2 years, Each sample was separated into 2-11 plant parts, depending on species and stage of growth, and for each part the in-vitro true dry matter digestibility, digestibility of cell wall and percentages of cell content, nitrogen, water-soluble carbohydrate and lignin were determined. Green leaflets of all three species were consistently high in digestibility. The cell wall fraction of green leaflets was less digestible in red clover than in white clover and lucerne. The digestibility of the upper part of the stem of lucerne and red clover was high at the flower bud stage but declined considerably thereafter, whereas the digestibility of the lower part of the stem was much lower at the flower bud stage and declined relatively little. The green leaf stalks and inflorescences were generally rather less digestible than green leaflets and, in lucerne and red clover, more digestible than stems. In white clover the inflorescence stalks were less digestible than the leaf stalks. The digestibility of plant parts was strongly correlated with the proportion of cell content, and less strongly with the digestibility of cell wall. The proportion of cell content was positively correlated with N%, and the digestibility of cell wall was negatively correlated with the proportion of lignin in cell wall.

SUMMARYTrue dry matter digestibility, proportion of cell content, digestibility of cell wall, N and water-soluble carbohydrate were determined in eight related grasses in February, June and September in each of three years. In a separate experiment, true dry matter digestibility, proportion of cell content and digestibility of cell wall were determined in five plant parts of vegetative tillers of two grasses in February, April, June, August, October and December in each of two years.Perennial ryegrass (Lolium perenne) was the most digestible grass and tall fescue (Festuca arundinacea) and Westerwolds ryegrass (Lolium multiflorum var. Westerwoldicum) were the least digestible. Italian ryegrass (Lolium multiflorum) was high in proportion of cell content, but not in digestibility of cell wall. Meadow fescue (Festuca pratensis) was high in digestibility of cell wall, but low in proportion of cell content. Perennial ryegrass and hybrid ryegrass were high, and tall fescue low, in both proportion of cell content and digestibility of cell wall. Ryegrasses were lower than fescues in concentration of N and higher than the fescues in water-soluble carbohydrate; ryegrass × meadow fescue hybrids were intermediate between the parent species in N and watersoluble carbohydrate.Grass sampled in June and August was lower in both proportion of cell content and digestibility of cell wall than grass sampled at cooler times of year. Grass sampled in February was high in proportion of cell content and N, intermediate to high in digestibility of cell wall and intermediate in water-soluble carbohydrate.Expanding leaf blades of perennial and Italian ryegrass were higher than expanded blades and sheaths in digestibility of cell wall and similar to expanded blades and higher than sheaths in proportion of cell content. Cell wall was less digestible in the tip than in the middle and basal portions of expanded leaf blades.

Italian ryegrass (cv. S22) and perennial ryegrass (cv. S24) were sampled duringprimary growth at head emergence, pollen shedding and seed shedding in each of 2 years. Each sample was separated into approximately 19 plant parts and for each part the true dry matter digestibility, digestibility of cell wall and percentages of cell content, nitrogen, water-soluble carbohydrate and lignin were determined. Trends reported in the literature, based on a smaller number of plant parts, were generally confirmed, but were shown to conceal appreciable variation. Thus, while, on average, stem declined in digestibility faster than leaf sheath, the upper sheaths declined in digestibility faster than the lower internodes and nodes; similarly, the proportion of cell content declined at a rather similar rate to digestibility in leaf blade and much less than digestibility in stem but the position was reversed between pollen shedding and seed shedding in the case of dead leaf blade and internodes. While the N content of the inflorescence as a whole did not decline greatly with advancing maturity, the N content of the rachis was nearly halved. The upper part of the crop was more digestible than the lower part at head emergence, but not at seed shedding. Italian ryegrass had a higher proportion of cell content than perennial ryegrass in all types of plant part. The digestibility of cell wall declined with advancing maturity in all the plant parts examined. The lower portions of internodes had a higher proportion of cell content than the upper portions. There were gradients from top to bottom of the plant, e.g. percentage N declined and percentage water-soluble carbohydrate increased from top to bottom.