Introduction. Nephrotoxicity is one of the important side effects of anthracycline antibiotics. The aim of this study was to investigate the effects of nicotinamide (NAD), an antioxidant agent, against nephrotoxicity induced by doxorubicin (DXR).
Methods. The rats were divided into control, NAD alone, doxorubicin (20 mg/kg, i.p.) and DXR plus NAD (200 mg/kg, i.p.) groups. At the end of the 10th day, kidney tissues were removed for light microscopy and analysis. The level of tissues' catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), inducible nitric oxide (iNOS) and endothelial nitric oxide (eNOS) activities were determined. Results. The activities of CAT, GPx, and GSH were decreased, and Po was increased in renal tissue of doxorubicin group compared with other groups. The tissue of the doxorubicin group showed some histopathological changes such as glomerular vacuolization and degeneration, adhesion to Bowman's capsule and thickening and untidiness of tubular and glomerular capillary basement membranes. Histopathological examination showed that NAD prevented partly DXR-induced tubular and glomerular damage. Conclusions. Pretreatment with NAD protected renal tissues against DXR-induced nephrotoxicity. Preventive effects of NAD on these renal lesions may be via its antioxidant and anti-inflammatory action.
Objective. The aim of this study was to investigate the efficacy of beta-aminopropionitrile (BAPN) and prednisolone on the prevention of esophageal damage and stricture formation after caustic esophageal burn. Method. Twenty-eight rats were divided into four equal groups. In groups 1, 2, and 3, caustic esophageal burns were generated by applying NaOH to the 1.5 cm segment of the abdominal esophagus. Group 4 was for the sham. Normal saline to group 1, BAPN to group 2, and prednisolone to group 3 were administered intraperitoneally as a single daily dose. Results. Treatment with BAPN decreased the stenosis index (SI) and histopathologic damage score (HDS) seen in caustic esophageal burn rats. The SI in group 4 was significantly lower compared with groups 1, 2, and 3. Group 2 had the minimum SI value in corrosive burn groups. The differences related to SI between groups 1, 2, and 3 were not statistically significant. The HDS was significantly lower in group 4 compared with groups 1, 2, and 3. The HDS in group 2 was significantly lower compared with groups 1 and 3. Conclusion. This study demonstrated that BAPN was able to decrease the development of stenosis and tissue damage better than prednisolone.
Background
Abdominal compartment syndrome (ACS) causes severe pathology in the cardiovascular, renal and pulmonary systems. Recent studies showed that pentoxifylline (PTX) has effects on increasing tissue oxygenation, healing capillary refill and reducing superoxides and hydroxyl radicals by inhibiting xanthine oxidase. In this study, our aim was to study the effects of PTX on free oxygen radicals and oxidative damage in rats with ACS model.
Materials and methods
ACS model was created in 32 male Wistar-Albino-rats, which were randomized into one of the four study groups: Group A (n:8), having ACS; Group B (n:8), having ACS and receiving PTX (50 mg/kg/day) intraperitoneal for 10 days; Group C (n:8), receiving PTX (50 mg/kg/day) intraperitoneal for 10 days without having ACS; Group D (n:8), having no ACS and not receiving PTX. On the 11th day blood samples were collected to measure alanine-amino-acid-transferase (ALT) and aspartate-amino-acid-transferase (AST) in the heart, malondialdehyde (MDA), myeloperoxidase (MPO) and glutathione (GSH) in the liver, lung and small bowel. Histopathologic injury scoring was done.
Results
Groups were compared in pairs. Group A compared to Group B: ALT increase, liver MDA, lung GSH and MPO decrease were statistically meaningful in Group B. Group A compared to Group C: ALT and liver MPO decrease and liver MDA increase were statistically meaningful in Group A. Group B compared to Group C: ALT increase, MDA and GSH decrease in the lung were statistically meaningful in Group B. Group B compared to Group D: ALT and MPO increase in the small bowel and MPO decrease in the lung were statistically meaningful in Group B. Group A had the highest histopathologic injury scoring.
Conclusion
Histopathologically confirmed pentoxifylline was effective in the treatment of ACS in these rat models.
Highlights
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