The ethanolic extract of strawberry (Fragaria. ananassa.) fruits were analyzed for total antimicrobial activity against four different strains of Staphylococcus aureus, Streptococcus pneumoniae, Proteus.vulgaris and E.Coli. In addition, fruits were analyzed for Antifungal activity against Asparagillus.Niger and Candida albicans.. Total anthocyanin content ethanolic extract of strawberries have enough antimicrobial spectra and there was no inhibition against fungi Aspergillus niger and Candida albicans. The ethnolic extracts were also analysed by U.V.Spectrophotometer for the determination of anthocynin content. Total anthocyanin content of increased with maturity of fruits. The results showed a linear correlation between ethanolic extract of fruits for its antimicrobial activity. Moreover ripen berries have more activity than the normal unripen fruits.. However, there was no inhibition against fungi Aspergillus niger and Candida albicans. The minimum inhibitory concentration values were 12.00, 12.50, and 11.00 mm (P<0.05) against S.aureus, Streptococcus pneumoniae, Proteus.vulgaris and E.coli respectively. The activity was tested against three different concentrations such as 1000, 800,500 & 100ug/ml. The minimum inhibitory concentration was also found at ethanolic extract at 100ug/ml.
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