ABSTRACT. Two new forms of Pogonia different from P. japonica and P. minor were found in Japan: One was called 'Miyamatokisou' collected in Mt.Hakusan and the other one was still unnamed and collected in Higashi-Hiroshima City, Hiroshima Prefecture. The karyotype of Pogonia called 'Miyamatokisou' was 2n=18 and consisted of 18 median-centromeric chromosomes but was a little bit different from that of P. minor (2n=18). That of Pogonia collected Higashi-Hiroshima-City was 2n=20 that was consisted of 16 median-centromeric chromosomes and four terminal-centromeric chtomosomes, and similar to that of P. japonica (2n=20). Origin and relationships of those two new forms were analyzed by RAPD (the randomly amplified polymorphic DNA) technique: According to the results of RAPD obtained here, Pogonia called 'Miyamatokisou' and Pogonia from Higashi-Hiroshima City had different DNA fragment-patterns from P. japonica, P. minor and their F 1 hybrids. Additionally, Pogonia called 'Miyamatokisou' was different from Pogonia originated from Higashi-Hiroshima City although their flower shape was similar to each other. It was confirmed that newly naturally developed Pogonia from P. japonica and P. minor existed in nature according to the RAPD analysis.
ABSTRACT:Only the two species of Pogonia, the Orchidaceae, such as P. japonica Rchb. f. and P. minor (Makino) Makino are distributed in Japan but currently newly appeared only one or two individuals so-called P. 'Miyamatokisou' nomen nudum occurred in two locations. Genic relationships among these three taxa of Pogonia were studied with respect to ITS (Internal Transcribed Sequences). Thus, repeatable DNA polymorphisms were found among 20 ISSR primers studied. According to genetic distances analyzed, a group of P. japonica and P. minor and the other group of two strains of P. 'Miyamatokisou' nomen nudum were isolated. Although these two strains of P. 'Miyamatokisou' were distantly distributed but were genetically similar to each other. The ISSR domain of P. 'Miyamatokisou' were different from those of P. japonica and P. minor, respectively. It was suggested that certain edge effects along with the distribution of those two species might perform their polymorphisms and got a new speciation to perform P. 'Miyamatokisou'. Depending on determination of ITS, the relationship between P. 'Miyamatokisou' and the two species of Pogonia were not determined. While the species of Pogonia have been recorded morphologically highly variable, the present research also concluded that those taxa studied here suggested highly variable.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.