Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200 µg/mL) were tested in MTT assay and cell proliferation was verified at 100 µg/mL after 24 h and at 25, 50, and 100 µg/mL after 48 h of extract exposure. Evaluation of antioxidant activity was performed at 0.5, 5, 25, 50, and 100 µg/mL HEJFP concentrations. Cell treatment with HEJFP at 25, 50, and 100 µg/mL for 24 h followed by H2O2 exposure for 3 h showed a strong cytoprotective effect. In vitro scratch wound healing assay indicated that none of tested HEJFP concentrations (0.5, 5, 25, 50, and 100 µg/mL) were capable of increasing migration rate after 12 h of incubation. These results demonstrate a positive effect of HEJFP on the wound healing process on L929 fibroblasts cell line, probably due to the antioxidant activity exhibited by phytochemicals in the extract.
O artigo tem como objetivo apresentar a experiência da Vivência em Agricultura Familiar, disciplina curricular obrigatória do curso de graduação em Agronomia da Universidade Federal de Santa Catarina, e caracterizar a imersão na realidade social rural enquanto metodologia de ensino. Insere-se na perspectiva analítica que considera teoria e prática como construtos inseparáveis no processo de ensino e aprendizagem e tem como base empírica os dados qualitativos coletados durante os processos coletivos de avaliação da etapa de campo da disciplina. Como principal resultado, desvela-se que a imersão na realidade rural produz múltiplos aprendizados, alguns para além dos objetivos da disciplina, sendo a relação teoria e prática o disparador de processos reflexivos sobre a ação e o efetivo papel dos futuros engenheiros/as agrônomos/as.
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