Treatment of Bacillus megaterium cell suspensions with 12 /μmol/1 chlorhexidine diacetate for 5 min led to an approximate 50%, reduction in viability when plated onto tryptone soya agar (TSA). Fifty percent of the surviving fraction were unable to form colonies on TSA containing 5.5% w/v KCI. Such loss of KCl tolerance is indicative of membrane damage, and was recovered within 30 min of incubation in tryptone soya broth (TSB). Multiplication of the damaged organisms did not recommence in this medium until after 60 min. Inclusion of inhibitors of respiration, and of protein, RNA and DNA synthesis in the TSB recovery medium did not significantly affect either the rate or extent of the recovery of KCl tolerance by damaged organisms.
Synthesis and Biological Evaluation of N-Salicyloyl-N-benzyl Thiourea and 2,2-Dimethyl-4-oxo-6-methoxy Benzo-1,3-dioxin. -The title compounds (V) and (VIII) are prepared as outlined in the reaction scheme. Heating of salicyloyl chloride (I) and ethyl thiocyanate (II) yields the benzoxazine (III) which on treatment with benzylamine (IV) in refluxing dioxane gives (V). Reaction of the 4-methoxy analogue (VI) with acetone (VII) leads to (VIII). The new compounds are screened for their biological activity. -(RASHAN, L. J.; AL-RAWI, J. M. A.; NADIR, M. T.; AWNI, L.; Farmaco, Ed. Sci. 46 (1991) 5, 677-683; Dep. Biol. Coll. Educ., Dep. Chem., Coll. Sci., Univ., Mosul, Iraq; EN)
Allwood, 1976). This a c t i o n bas been a t t r i b u t e d t o p e r t u r b a t i o n of t h e cytoplasmic membrane, l e a d i n g t o l o s s of e s s e n t i a l i n t r a c e l l u l a r small molecular weight m e t a b o l i t e s , such as potassium (Lambert & Hammond, 1973). of s a l t s , e s p e c i a l l y tk.ose of potassium, a t c o n c e n t r a t i o n s i s o t o n i c w i t h t h e i n t r a c e l l u l a r environment might be expected t o reduce t h e r a t e of potassiumi o n leakage and perhaps decrease t h e a c t i v i t y of t h e s e a g e n t s .
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