Thyroid hormones (THs) regulate a multitude of developmental and metabolic processes, which are responsible for vertebrate development, growth, and maintenance of homeostasis. THs also play a key role in neurogenesis of vertebrates and thus affect eye development, which is vital for foraging efficiency and for effective escape from predation. Currently, there are no validated test guidelines for the assessment of TH system-disrupting chemicals (THSDCs) in fish. Consequently, the present study was designed to demonstrate the suitability of novel thyroid-related endpoints in early life-stages of fish. Embryos of a transgenic zebrafish (Danio rerio) line expressing the reporter gene tg:mCherry in their thyrocytes were used to investigate the effects of the environmental THSDCs triclosan (TCS, antibacterial agent) and benzophenone-2 (BP-2, UV filter) on thyroid follicle and eye development. Both BP-2 and TCS caused thyroid follicle hyperplasia in transgenic zebrafish, thus confirming their role as THSDCs. The effect intensity on follicle size and fluorescence was comparable with a 1.7-fold increase for BP-2 and 1.6-fold for TCS. Alterations of the cellular structures of the retina indicate an impact of both substances on eye development, with a stronger impact of TCS. With respect to guideline development, results provide further evidence for the suitability of morphological changes in thyroid follicles and the eyes as novel endpoints for the sensitive assessment of THSD-related effects in fish.
We show that any homogeneous initial data set with Λ < 0 on a product 3-manifold of the orthogonal form (F × S1, a0 2dz2 + b0 2σ, c0dz2 + d0σ), where (F, σ) is a closed 2-surface of constant curvature and a0, . . . , d0 are suitable constants, recollapses under the Einstein-flow with a negative cosmo- logical constant and forms crushing singularities at the big bang and the big crunch, respectively. Towards certain singularities among those the Kretschmann scalar remains bounded. We then show that the presence of a massless scalar field causes the Kretschmann scalar to blow-up towards both ends of spacetime for all solutions in the corresponding class. By standard arguments this recollapsing behaviour extends to an open neighborhood in the set of initial data sets and is in this sense generic close to the homogeneous regime.
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