We assessed the effects of freezing and refrigeration over long periods on the microbiological quality of sheep milk. The raw milk was frozen in 1-L plastic bags or 5-L milk buckets and, after 1 mo, thawed at 7 or 25°C. We evaluated these samples immediately after thawing (d 0) and after 1 d of storage at 7°C. Furthermore, we stored fresh raw milk at 7°C for 10 d in the same packages and in a bulk milk cooler at 4°C (adding 10% of fresh raw milk daily). The total bacterial, total psychrotolerant, and proteolytic psychrotolerant counts were evaluated before and after thawing (for previously frozen milk) and daily (for refrigerated milk). The frozen-thawed milks showed no significant increase in bacterial counts immediately after thawing for all samples (<0.7 log cfu/mL), but only the samples packaged in 1-L bags and thawed at 7°C remained microbiologically adequate after 1 d of storage. Findings of the refrigerated samples were modeled using a modified Gompertz equation, obtaining a lag phase of around 0.5 (5-L bucket), 2.6 (1-L bag), and 7.0 (bulk milk cooler) d for total bacterial and total psychrotolerant counts. Maximum growth rates (µ max ) were 1.0 and 1.0 (5-L bucket), 1.2 and 1.3 (1-L bag) and 3.0 and 1.5 (bulk milk cooler) ln(cfu/mL) per day for total bacteria and total psychrotolerant counts, respectively. Compared with total bacteria and total psychrotolerant bacteria, psychrotolerant proteolytic bacteria grew slowly, reaching unacceptable counts only after 9 to 10 d of storage. The studied methods are interesting alternatives for preserving raw sheep milk on smallholdings.
The impact of microbial growth on the physicochemical parameters of sheep milk was evaluated. The total bacterial counts (TBC) showed a lag phase of 4 h and µ-max of 0.4 log/h. After 8.4 h, the TBC reached the limit established by European Community, pH had a reduction of 0.04, acidity had an increase in 0.04%, and no changes were observed in the ethanol stability. The milk became thermally unstable after 17 h (10 9 cfu/mL), when the pH was 5.71, acidity was 0.36%, and ethanol stability was 21.3%. These results highlighted the extreme stability of sheep milk protein and that acidity may be the best physicochemical parameters to predict the quality of sheep milk.
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