Specimens of Arapaima gigas from Jamari River (RO) were cytogenetically analyzed. A diploid number of 2n=56 chromosomes was found (28m-sm + 28st-a). Secondary constrictions were observed on the short arms of chromosome 3. Nucleolar Organizer Regions (NORs) were detected at the subterminal region on short arms of the third chromosomal pair by both silver nitrate staining and FISH with 45S rDNA probe, being equivalent to secondary constrictions. The ribosomal sites were also characterized by size heteromorphism and presence of CMA 3 + /DAPI -blocks. The constitutive heterochromatin was located at pericentromeric region of some chromosomes. After sequential Cbanding and base-specific fluorochromes staining, most of the heterochromatins proved to be neutral, i.e., with similar amounts of AT and GC bases. Nonetheless, some heterochromatic regions were marked by GC-specific fluorochromes in one chromosomal pair and by AT-specific fluorochrome staining on two pairs. The present data are in agreement with previous reports in populations from Araguaya River, indicating that conserved cytogenetic features are present in this important fish species.
Cytogenetic and morphometric analyses were carried out in Hoplias aff. malabaricus specimens from six distinct populations from the lower Paranapanema River basin, located between the states of Paraná and São Paulo, Brazil. Measurements were also taken from a specimen collected in Surinam. In a population from a fish farm station at Universidade do Norte do Parana (EPUNOPAR), two sympatric cytotypes (2n = 40 and 2n = 42 chromosomes) are found. A population from a fish farm station at Universidade Estadual de Londrina (EPUEL) shows 2n = 42 meta-submetacentric chromosomes for males and females with a simple sex chromosome system of XX/XY type. Populations from the Vermelho and Rancho Alegre Rivers, Três Bocas Stream and Paranapanema River have 2n = 39 chromosomes in males and 2n = 40 chromosomes in females, showing a multiple sex chromosome system of X(1)X(1)X(2)X(2)/X(1)X(2)Y type. Twenty morphological variables were studied. These measurements were used for an analysis of the canonical variables and standard analysis of proportional measurements. The most variable measurements among the specimens are the maxilla length (MXL) and the pre-dorsal distance (PDD). Analysis of canonical variables indicates three distinct groups in the first canonical axis formed by: (1) Três Bocas Stream, (2) Rancho Alegre + Vermelho River + EPUNOPAR and (3) EPUEL + Paranapanema River. This axis retained 79.4% of information from the original matrix. Analysis of morphometrics reveals differences among populations from the Paranapanema River basin and between these and the specimen from Surinam. The morphometric and cytogenetic differences among the studied populations suggest a species complex.
ResumoForam utilizados 72 juvenis de tilápia do Nilo, Oreochromis niloticus, sexualmente revertidos, com peso médio inicial de 37,27 ± 4,92 g, distribuídos num delineamento inteiramente casualizado, durante 112 dias. Este trabalho avaliou o efeito da introdução de gaiolas com cultivo de tilápias alimentados com 0% (controle), 20%, 40% e 60% (grupos teste) de levedura em substituição à ração de fontes tradicionais sobre o peso, a sobrevivência e conversão alimentar e também verificou se há diferença entre a função perda de Taguchi para a produção total, para os peixes mortos e descartados e as quatro rações balanceadas. A análise de variância foi aplicada mostrando que não houve diferenças estatísticas (p > 0,05) entre os efeitos dos tratamentos sobre a sobrevivência e conversão alimentar, mas houve efeito quadrático sobre o ganho de peso das tilápias (Y = 73,39 + 0.173X -0.0034X2; R 2 = 0,9986), concluindo que o melhor nível de inclusão de levedura como fonte protéica na dieta para juvenis revertidos é de 25,44%. As perdas de Taguchi para os custos de produção e para os peixes mortos nos tratamentos T 2 = 20%, T 3 = 40% e T 4 = 60% foram menores que o grupo controle (T 1 = 0%). Este resultado confirma o direcionamento da inclusão de levedura de destilaria em substituição a ração de origem tradicional. Palavras-chave: Estatística, sobrevivência, tratamento, variância AbstractThe experiment was based on observations of 72 juveniles of tilapias of Nile, Oreochromis niloticus, sexually reverted, with an initial medium weight of 37.27 ± 4.92 g, distributed in a completely randomized design for 112 days. This work has evaluated the effect of the introduction of cages with cultivation of tilapia fed 0% (control), 20%, 40% and 60% (groups test) of yeast replacing the traditional sources of diet on weight, survival and feed conversion and also there is no difference between the loss function of Taguchi to the total for the dead fish and discarded and the four balanced rations. The analysis of variance was applied showing that there were no statistical differences (p> 0.05) between the effects of treatments on the survival and feed conversion, but there was a quadratic effect on weight gain of tilapia (Y = 73.39 + 0.173X -0.0034X2, R2 = 0.9986), concluding that the best level of inclusion of yeast as protein source for juvenile is reversed 25.44%. Taguchi's loss for the cost of production and for the dead fishes in the treatment T 2 = 20%, T 3 = 40% and T 4 = 60% were lower the group control (T 1 = 0%). This result confirms the direction of inclusion of distillers yeast in place of traditional feed source.
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