Fluid milk and its derivatives are important dietary ingredients that contribute to daily nutrient intake of the modern Homo sapiens. To produce milk that is healthier for human consumption, the present study evaluated the effect of adding soybean oil and linseed oil in the diet of lactating cows. The fatty acid profile of milk, milk composition, and the blood parameters of cows were evaluated. Eighteen Holstein cows were distributed in a replicated Latin square design and distributed according to the following treatments: 1) Control (CC): traditional dairy cow diet, without addition of oil; 2) Soybean oil (SO): 2.5% addition of soybean oil to the traditional diet, as a source of omega-6; 3) Linseed oil (LO): 2.5% addition of linseed oil in the diet as a source of omega-3. Milk production was not affected, but oil supplementation decreased feed intake by 1.93 kg/cow/day. The milk fat percentage was significantly lower when cows were supplemented with vegetable oil (3.37, 2.75 and 2.89% for CC, SO and LO, respectively). However, both soybean and linseed oils decreased the concentration of saturated fatty acids (66.89, 56.52 and 56.60 g/100g for CC, SO and LO respectively), increased the amount of unsaturated fatty acids in milk (33.05, 43.39, and 43.35 g/100g for CC, SO and LO respectively) and decreased the ratio between saturated/unsaturated fatty acids (2.12, 1.34, and 1.36 for CC, SO and LO respectively). Furthermore, SO and LO increased significantly the concentration of monounsaturated fatty acids (29.58, 39.55 and 39.47 g/100g for CC, SO and LO respectively), though it did not significantly alter the level of polyunsaturated fatty acids in milk fat (3.57, 3.93 and 3.98 g/100g for CC, SO and LO respectively). Supplementation with LO enhanced the concentration of omega-3 fatty acids on milk (0.32, 0.36, and 1.02 for CC, SO and LO respectively). Blood variables aspartate aminotransferase, gamma glutamyl transferase, urea, albumin, creatinine and total proteins were not altered. On the other hand, total cholesterol, HDL and LDL were greater in the group supplemented with vegetable oils. Supplementation with vegetable oils reduced the dry matter intake of cows, the fat content of milk, and improved saturated/unsaturated fatty acid ratio of milk fat. Compared to the SO treatment, animals fed LO produced milk with greater content of omega-3, and a more desirable omega-6/omega-3 ratio on a human nutrition perspective. Thus, the inclusion of SO and LO in the diet of lactating dairy cows makes the milk fatty acid profile nutritionally healthier for the human consumption.

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The study aimed to evaluate the supplementation of gilts with cow’s milk naturally enriched with n-3 and n-6 polyunsaturated fatty acids (PUFA) on reproductive outcomes, and the serum biochemical and FA profile of swine females and their offspring. During 316 days, 30 gilts were distributed into three groups: (1) Control, fed a basal diet + milk from cows without oil; (2) n-3, fed a basal diet + milk from cows fed a diet enriched with linseed oil; (3) n-6, fed a basal diet + milk from cows fed a diet enriched with soybean oil. The gilts receiving the diets containing PUFA had higher serum urea and very-low-density lipoprotein levels and lower serum total protein and low-density lipoprotein levels compared to the Control group. Females supplemented with n-3 presented higher serum palmitic acid and γ-linolenic acid levels than those fed n-6. Piglets from the Control group were heavier at birth than those from females supplemented with enriched milk. The piglets from females receiving enriched milk had 140 g higher body weight from 1 to 21 days old compared to the Control group, and greater average daily weight gain from 7 to 14 days old. The serum eicosapentaenoic acid level of piglets fed n-3 was 69% higher than those fed n-6, which reduced the AA/EPA ratio. Gilts supplemented with PUFA-enriched cow’s milk showed changes in their serum palmitic and γ-linolenic acid levels, in addition to improved performance, EPA concentration and consequently reduced AA/EPA ratio in their piglets, demonstrating beneficial results for their progeny.

Pegbovigrastim is a modified form of the naturally-occurring bovine granulocyte colony-stimulating factor. At the time of the study, pegbovigrastim was commercially available under veterinary prescription as Imrestor (Elanco Animal Health, Greenfield, Indiana). According to the product label, when the product is applied 7 days before calving and again at the day of calving, clinical mastitis incidence decreases by 28% in the first 30 days of lactation in dairy cows and heifers. The objective of this study was to further demonstrate the effectiveness of Imrestor at reducing clinical mastitis incidence, and to demonstrate the effectiveness at reducing metritis incidence in the first 30 days of lactation on a commercial dairy operation.

The objective was to evaluate the influence of cell-mediated immune response classification on performance of dairy cattle experiencing conditions of heat stress on pasture. On d 0, pregnant, lactating dairy cows (n = 27; 18 Holstein and 9 Jersey) received a subcutaneous sensitization dose of killed Candida albicans (CA; 0.5 mg) with Quil-A adjuvant (0.75 mg) in 2.5 mL of PBS. Tail skin fold thickness (SFT) was measured with a Harpenden caliper on d 14 immediately following an intradermal injection of killed CA (0.1 mg in 0.5 mL of PBS) in the right tail skin fold and a control injection (PBS) in the left tail skin fold. On d 15, change in SFT was measured and used to assign cell-mediated immune response classifications (CMIR) as High (HR; >0.5 SD above the mean; n = 9), Intermediate (IR; within 0.5 SD above or below the mean; n = 10), or Low (LR; >0.5 SD below the mean; n = 8). Cows from each CMIR were assigned to 1 of 3 environmental conditions (EC): No Abatement (no heat abatement provided; n = 9), Shade (portable shade structure; n = 9), or Sprinklers (polyvinyl chloride sprinkler system; n = 9) for a 38-d period (July 1, 2019 to August 8, 2019). Daily Milk Yield (DMY), Reticulorumen Temperature (RT), Heat Stress Score (HSS; 0 = respiration rate of < 80 breaths/min to 4 = moribund, labored breathing), and instances of Mouth Open (MO), Tongue Out (TO), and Drool were recorded daily. Respiration rate (RR; breaths/min) was recorded 3 times/day (morning, midday, and late afternoon) and averaged on Monday, Wednesday, and Friday weekly. Body Condition Score (BCS; 1 = emaciated to 5 = obese) was recorded weekly. Data were analyzed using MIXED procedures of SAS specific for repeated measures with CMIR, EC, Breed, Time and 2-way interactions as fixed effects and Pen as random. Low exhibited the lowest HSS and BCS, but the greatest MO and TO instances (P < 0.02). High exhibited the greatest instances of Drool (P < 0.02). Heat stress score, MO, and Drool were greatest in the No Abatement group (P < 0.01). Respiration rate was greatest in the No Abatement and Shade groups relative to Sprinklers (P < 0.01). Daily milk yield, RT, and Drool were increased whereas HSS and RR were decreased in Holstein relative to Jersey cows (P < 0.01). Interactions (P < 0.05) included: CMIR by EC for DMY, HSS, RR, MO, TO, and Drool; CMIR by Breed for RT, DMY, and BCS; EC by Breed for RT, DMY, HSS, and RR; and EC by Time for DMY, HSS, BCS, RR, MO, and TO. There was an effect of Time for all variables (P < 0.05). Preliminary results suggest an influence of cell-mediated immune response classification on performance of dairy cattle experiencing conditions of heat stress and warrant future studies with an increased number of animals.

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