Crystals were purified from four serotypes of the insect pathogen Bacillus thuringiensis. Crystals from these serotypes were similar in amino acid and N-terminal analyses, but differed in their toxicity to two species of Lepidoptera and in their immunological properties. Toxic polypeptides were obtained following trypsin digestion of solutions of the crystals. In two strains (serotypes 3 and 9) this fraction contained only one polypeptide. Similar results were obtained when dissolved crystals were digested with other proteolytic enzymes or with gut contents from Pieris brassicae. The trypsin-resistant polypeptide was further purified by gel and ion-exchange chromatograhy and had a molecular weight of about 70,000, estimated by gel chromatogrpahy and gel electrophoresis. No evidence was obtained for a toxin of lower molecular weight. This purified toxin accounted for most, if not all, of the toxic activity originally present in the crystal solution and was active by injection and ingestion. The purified toxic fraction from serotype 1 appeared to contain two polypeptides, one of which corresponded to that found with serotypes 3 and 9. There were no major differences in the composition of crystals from different serotypes of B. thuringiensis and it is concluded that the trypsin-resistant polypeptide represents the active insecticidal toxin of the crystal.

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AimsTo review our assessment of infants discharged directly from the emergency department who had presented with an injury under 1 year of age. To review adherence of usage of the injury under 1 proforma. To ascertain the number of children who had more than 1 injury under 1 year of age. To compare results with the first loop of the audit in August 2014MethodsThis was a retrospective audit reviewing case notes of all infants who presented to the emergency department with an injury during the 6 month period of May- October 2016. We compared our results to a smaller audit completed in 2014.ResultsA total of 440 patients under 1 were discharged following a presentation due to injury during the 6 month period. Of the 440 patients 50 (11.4%) did not have correct proforma completed (table 1).Abstract G131(P) Table 1Total injuries under 1 and further re-attendances over 12 months of age Number % of total 1 injury<12 months of age only 390 88.6% 2 injuries<12 months of age 48 10.9% 3 injuries<12 months of age 2 0.5% 0 further injuries over 12 months* 362 82.2% 1 further injury over 12 months of age* 72 16.4% 2 further injuries over 12 months of age* 4 0.9% 3–4 further injuries over 12 months of age* 2 0.5% *Patients followed up for 12 months following initial presentationDiscussionFrom our previous 2014 data there has been an improvement in use of the injury under 1 proforma; 88.6% compliance versus 70% in 2014. The re-presentation rate increases with age which is expected given increased mobility over 1. The patient who had multiple (4) re-presentations was referred to social work resulting in extra parental support. One patient who had 2 injuries under 1 subsequently presented with a burn age 16 months. There were serious safety concerns with the result being the child was placed in kinship care.

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