We examined the development of a 33-year experiment in spruce–fir stands that received nine herbicide treatments (applied aerially in 1977), with and without precommercial thinning (PCT) (applied in 1986). We tested two commonly held assumptions about the long-term effects of herbicide and PCT in mixedwood stands managed for softwoods: (i) herbicide release produces stands dominated by softwoods and (ii) PCT promotes both softwood dominance and merchantable volume. All herbicides were effective at releasing balsam fir ( Abies balsamea (L.) Mill.) and spruce ( Picea spp.) from overtopping shrubs and hardwoods that had naturally regenerated following clearcut harvesting in 1970. Glyphosate (Roundup), triclopyr amine (Garlon 3A), 2,4,5-T, and a mixture of 2,4,5-T and 2,4-D herbicide treatments were all effective at shifting long-term species composition to softwood dominance after 30 years, irrespective of the rates applied. Herbicide-treated stands that did not receive PCT were overstocked with softwoods for at least 24 years and produced less than half of the merchantable softwood volume by 40 years than herbicide-treated stands that received PCT. Intolerant hardwoods dominated untreated stands for all 33 years of the experiment. When unsprayed plots received PCT, however, they produced stands at 40 years that were compositionally and structurally indistinguishable from those that had received both herbicide and PCT treatments. Results from this experiment clearly demonstrated that early herbicide application, regardless of type and rate of herbicide, created softwood-dominated mixedwood stands over the long term and that PCT more than doubled merchantable softwood volume within 25 years of application.

No abstract

Does species shade tolerance regulate natural regeneration abundance and composition when partial harvestings (≤ 80% of basal area removal) are operated on a landscape-scale? We examined this question using 835 permanent plots located across forested landscapes of Maine, USA. These plots were surveyed for regeneration growth, mortality, and recruitment before and after treatment application (i.e., partially harvested and unharvested). Our results showed that relative to unharvested stands, high-intensity partial harvesting (41-80% of basal area removal) increased the number of seedlings (<2.5 cm at dbh) recruited to saplings (2.5-12.69 cm at dbh) and sapling diameter growth irrespective of species shade tolerance over a 15-year, post-treatment period. However, high-intensity partial harvesting increased sapling mortality during the initial five years since harvesting whereas low-intensity partial harvesting (5-40% of basal area removal) maintained the natural regeneration dynamics (growth, recruitment, and mortality) of unharvested stands. We found that harvesting intensity, basal area and seedling density by shade-tolerance group before harvesting are more important attributes than species shade tolerance for determining the responses of natural regeneration to partial harvesting. The greater importance of pre-harvest stand attributes on post-harvest regeneration may suggest an integrated overstory and understory manipulation approach for attaining the desired regeneration composition.

No abstract

Question: Two questions about within‐stand spatial variability are addressed in this paper. How does species richness of tree regeneration respond to small‐scale ecological gradients, and what effect does natural Abies balsamea abundance have on the species richness of other tree regeneration? Location: A long‐term, gap‐silviculture experiment, Acadian mixed‐wood forest, Maine, USA. Methods: Eight stands treated with and without gap harvesting were sampled to capture sub‐stand heterogeneity of understorey tree regeneration concurrently with patterning of local stand conditions. Spatial and non‐spatial models were developed to test the relationships between two response variables [species richness of small (height ≥0.1 m, but <0.75 m) and large (height ≥0.75 m, but <1.4 m) regeneration] and five explanatory variables (depth to water table, percentage canopy transmittance, A. balsamea regeneration density, and overstorey basal area and species richness). Results: Despite high unexplained variance for all models, consistent associations among variables were found. Negative associations were found between: (1) the species richness of small regeneration and A. balsamea regeneration density and (2) the species richness of large regeneration and overstorey basal area. Positive associations were found between: (1) the species richness of small regeneration and both overstorey basal area and species richness and (2) the species richness of small and large regeneration and canopy transmittance. Conclusions: Promoting tree species diversity in Acadian mixed‐wood stands may not be achievable through the use of gap‐harvesting alone if the density of understorey Abies balsamea is not reduced either naturally or through silvicultural intervention.

In nuclear and radiological incidents, overexposure to ionizing radiation is life-threatening. It is evident that radiation depletes blood cells and increases circulating cytokine/chemokine concentrations as well as mortality. While microglia cells of female mice have been observed to be less damaged by radiation than in male mice, it is unclear whether sex affects physio-pathological responses in the bone marrow (BM) and gastrointestinal system (GI). We exposed B6D2F1 male and female mice to 0, 1.5, 3, or 6 Gy with mixed-field radiation containing 67% neutron and 33% gamma at a dose rate of 0.6 Gy/min. Blood and tissues were collected on days 1, 4, and 7 postirradiation. Radiation increased cytokines/chemokines in the femurs and ilea of female and male mice in a dose-dependent manner. Cytokines and chemokines reached a peak on day 4 and declined on day 7 with the exception of G-CSF which continued to increase on day 7 in female mice but not in male mice. MiR-34a (a Bcl-2 inhibitor), G-CSF (a miR-34a inhibitor), MAPK activation (pro-cell death), and citrulline (a biomarker of entro-epithelial proliferation), active caspase-3 (a biomarker of apoptosis) and caspase-1activated gasdermin D (a pyroptosis biomarker) were measured in the sternum, femur BM and ileum. Sternum histopathology analysis with H&E staining and femur BM cell counts as well as Flt-3L showed that BM cellularity was not as diminished in females, with males showing a 50% greater decline on day 7 postirradiation, mainly mediated by pyroptosis as indicated by increased gasdermin D in femur BM samples. Ileum injury, such as villus height and crypt depth, was also 43% and 30%, respectively, less damaged in females than in males. The severity of injury in both sexes was consistent with the citrulline and active caspase-3 measurements as well as active caspase-1 and gasdermin D measurements, suggesting apoptosis and pyroptosis occurred. On day 7, G-CSF in the ileum of female mice continued to be elevated by sevenfold, whereas G-CSF in the ileum of male mice returned to baseline. Furthermore, G-CSF is known to inhibit miR-34a expression, which in ileum on day 1 displayed a 3- to 4-fold increase in female mice after mixed-field (67% neutron + 33% gamma) irradiation, as compared to a 5- to 9-fold increase in male mice. Moreover, miR-34a blocked Bcl-2 expression. Mixed-field (60% neutron + 33% gamma) radiation induced more Bcl-2 in females than in males. On day 7, AKT activation was found in the ileums of females and males. However, MAPK activation including ERK, JNK, and p38 showed no changes in the ileum of females (by 0-fold; P &gt; 0.05), whereas the MAPK activation was increased in the ileum of males (by 100-fold; P &lt; 0.05). Taken together, the results suggest that organ injury from mixed-field (67% neutron + 33% gamma) radiation is less severe in females than in males, likely due to increased G-CSF, less MAPK activation, low miR-34a and increased Bcl-2/Bax ratio.

No abstract

No abstract