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Hedgehog signaling regulates neurogenesis in the larval and adult zebrafish hypothalamus 2. Abbreviated Title (50 character maximum) Hedgehog signaling and hypothalamic neurogenesis 3. List all Author Names and Affiliations in order as they would appear in the published article

24While neurogenesis in the adult hypothalamus is now known to be essential for proper function, 25the cell-cell signaling events that regulate neurogenesis in this evolutionarily conserved brain 26 region remain poorly understood. Here we show that Hedgehog (Hh)/Gli signaling positively 27 regulates hypothalamic neurogenesis in both larval and adult zebrafish and is necessary and 28 sufficient for normal hypothalamic proliferation rates. Hedgehog-responsive cells are relatively 29 rapidly proliferating pluripotent neural precursors that give rise to dopaminergic, serotonergic, 30and GABAergic neurons. in situ and transgenic reporter analyses revealed substantial 31 heterogeneity in cell-cell signaling within the hypothalamic niche,

Neuroscientists now routinely record the activity of large numbers of neurons at high temporal and spatial resolution. With these capabilities comes the promise of causally intervening during these recordings by perturbing neurons or changing experimental conditions, requiring tight integration between data acquisition, analysis, and manipulation. Unfortunately, solutions for real-time interventions are rare, difficult to design and implement, and remain largely unused. Here, we introduce improv, a software platform that allows users to flexibly specify and manage adaptive experiments to integrate data collection, preprocessing, visualization, and user-defined analytics. Using improv for streaming data analysis for two photon calcium imaging and behavior we demonstrate how access to online information can be used for automated, integrated experimentation.