All professionals in the hospital have a risk of stress, but nurses have a higher stress level. High levels of work stress also affect the performance, satisfaction, productivity, and caring behavior of nurses will be lower. If nurses experience work stress and are not managed properly, it can eliminate the care for patients, increase errors in patient care and endanger patient safety. This study aims to analyze the relationship of workload, interpersonal relationships, work safety and the level of dependence of patients with nurses work stress at Naimata General Hospital Kupang. This type of research is an analytic survey with cross sectional study design. The population in this study were 38 people with samples taken using the Total Sampling technique, the test used was a multiple linear regression test. T test results show there is a relationship between workload (p = 0.029 <α = 0.05), interpersonal relationship (p = 0.012 <α = 0.05) and work safety (p = 0,000 <α = 0.05) with stress nurse work. There was no relationship between the level of patient dependence (p = 0.281> α = 0.05) with nurses work stress.The results of the F test showed that together with the independent variables related to work stress nurses (p = 0,000 <α = 0.05) with a coefficient of determination of 62.5%. It is recommended for the hospital to conduct training for nurses on how to handle mental patients.
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