Introduction: Type 1 diabetes can lead to muscle apoptosis and a defect in the structure of the heart tissue. One of the main pathways of apoptosis is the pathway activated by the P53 and Caspase-3 proteins. Therefore, the aim of this study was to investigate the effect of 4 weeks of high intensity interval training on the content of P53 and Caspase-3 proteins in heart muscle tissue of Sprague-Dawley rats with type 1 diabetic rats. Methods: In this experimental study, twelve 2-month-old Sprague-Dawley male rats with mean weight of 300±20 gr were selected and after induction of diabetes through streptozotocin solution, randomly divided into 2 groups: experimental (n= 6) and control (n= 6). The training program consisted of 5 stages of 4-minute with an intensity of 85 to 95% of the maximum speed and 3-minute active rest periods with an intensity of 50 to 60% of the maximum speed. Proteins were measured by Western-Blot in vitro. SPSS software version 16 and independent t-test were used to analyze the data. Results: High intensity interval training resulted in a significant reduction in the P53 protein content of the training group compared to the control group (P=0.003). However, there was no significant change in the content of Caspase-3 in the activated form (P=0.71) and the initial form (P=0.15) in the training group compared to the control group. Conclusion: High intensity interval training, by reducing the content of the P53 protein and preventing the activation of the caspase-3 protein, may have inactivated the apoptosis pathway of these two proteins in heart cells in type 1 diabetic subjects.
Background The FOXO3a/Beclin-1 pathway is an important pathway in autophagy that can be impaired in diabetic patients who are prone to cardiomyopathy. Objective The aim of this study was to investigate the effect of an 8-week endurance training program on the content of FOXO3a and Beclin-1 proteins in the heart muscle tissue of rats with type 2 diabetes. Methods This experimental study was conducted on 12 male two-month-old Sprague Dawley rats with a mean weight of 270±20 g. After diabetic induction by streptozotocin and nicotinamide, rats were randomly assigned into two groups of diabetic-exercise (n=6) and diabetic-control (n=6). The diabeticexercise group received intervention 4 days per week, each session for 42 minutes at a speed of 10-30 m/m for 8 weeks, while the control group received no any training program. The rats did not receive any insulin treatment during the study. Collected data were analyzed using independent t-test at a significance level of P≤0.05. Findings No significant changes were observed in the content of FOXO3a (P=0.12) and Beclin-1 (P=0.34) proteins in the training group compared to the control group after intervention. Conclusion The endurance training can not affect the content of FOXO3a and Beclin-1 proteins. Therefore, it seems that endurance training may not affect autophagy signaling in the heart muscle of type 2 diabetic patients.
Introduction: The mTOR and SREBP1 proteins play an important role important in the regulation and metabolism of adipose tissue that can be activated through the mTORC1 pathway. The purpose of the present study was to investigate the effect of 8 weeks endurance training on the content of mTOR and SREBP1 proteins in subcutaneous fat tissue in obese type 2 diabetic male Sprague-Dawley rats. Methods: In this experimental study, 16 three-month-old Sprague-Dawley rats with a mean weight of 300±20 g were selected. After diabetic induction with Streptozotocin and Nicotinamide, rats were randomly assigned to two groups, diabetic training (n=8) and diabetic control (n=8). The training group trained for 4 days a week in accordance with the training program for 8 weeks, while the control group did not have any training program. The independent t-test in SPSS software ver. 19 was used to analyze the data. Results: There was a significant increase in the content of mTOR (p<0.0001) and SREBP1 (p<0.0001) proteins in the training group compared to control; The control group weight increased (p<0.003) and training group (p<0.002) significantly decreased at the end of the eighth week compared to the first week. The blood glucose increased in the control group (p<0.001) and decreased in the exercise group (p<0.0001) in the eighth week compared to the first week. Conclusion: Endurance training can adjust the weight, blood glucose and proteins content of mTOR and SREBP1; Therefore, endurance training can be an important factor in controlling and regulating fat tissue metabolism; this type of training can be effective for obese subjects with type 2 diabetes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.