In the course of investigations on yeast cell production and on the metabolisms of methanol, two new species, Torulopsis nagoyaensis and Hansenula ofunaensis, were found in the yeasts isolated from various natural sources in Japan. Taxonomical studies of these new species are described.Since OGATA et al. (1-3) first reported the assimilation of methanol by yeast as a sole source of carbon, many methanol-assimilating yeast strains have been isolated from natural sources or found in stock culture collections (4-12).We isolated many yeast strains which can grow well at 37° in methanol medium. Among them, 3 strains could not be identified with any known species described in the literature. Two of these strains belonged to the same species of the genus Torulopsis and the other to the genus Hansenula. This paper deals with the descriptions of these two new species.
MATERIALS AND METHODSMicroorganisms. Yeast strains, KM-1359, KM-1722, and KM-1833, were used in this study. They were isolated as yeasts assimilating methanol strongly from natural sources by methanol-enrichment culture method.Determination of yeasts. The experimental methods were mainly those described by LODDER and KREGER-VAN RI7 (13), LODDER et al. (14), and IIZUKA and GoTo (15).
RESULTS AND DISCUSSIONTwo strains, KM-1722 and KM-1833, reproduced by multilateral budding and did not form ballistospores, ascospores, teliospores, endospores, or arthrospores. The vegetative cells were not triangular or "ogival." Pseudomycelia were not formed. They did not produce a starch-like compound or strong acetic acid from 197
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