Reticulol, an inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase.

Furutani, Yoshio; Shimada, Masao; Hamada, Masa; Takeuchi, Tomio; Umezawa, Hamao

doi:10.7164/antibiotics.28.558

Cited by 23 publications

(13 citation statements)

References 6 publications

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“…The phosphodiesterase inhibitor, reticulol [2,3], was found in the culture broth of a strain of Streptoverticillium sp. NA-4803 [1].…”

Section: Discussionmentioning

confidence: 99%

“…Until now, the reticulol from Streptomyces rubrireticulae [2] and S. mobaraensis [4,5] has been reported as a phosphodiesterase inhibitor [3]; however, studies on its cytotoxicity against lung tumors and melanoma metastasis have not been reported. This paper is concerned with the in vitro cytotoxicity of reticulol produced from Streptoverticillium and its in vivo biological activity blocking lung metastasis by inhibiting the melanoma growth.…”

Section: Introductionmentioning

confidence: 99%

“…NA-4803 produces an antitumor antibiotic [1]. This compound was revealed to be reticulol (6,8-dihydroxy-7-methoxy-3-methylisocoumarin) [2][3][4][5] and showed a specific inhibitory activity against Trichophyton mentagrophytes ATCC 18749, which is a worldwide pathogenic fungus causative of nail mold disease in animals and humans [6], and a potent cytotoxicity against A427 ATCC HTB 53, a human lung tumor cell line, and B16F10 ATCC CRL 6475, a mouse melanoma cell line.…”

Section: Introductionmentioning

confidence: 99%

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Antitumor Efficacy of Reticulol from <i>Streptoverticillium</i> against the Lung Metastasis Model B16F10 Melanoma

et al. 2003

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Reticulol was isolated from the culture broth of the strain Streptoverticillium sp. NA-4803. Recticulol (M.W. 222.2) exhibited a potent in vitro cytotoxicity against A427, a human lung tumor cell line, and B16F10, a mouse melanoma cell line. In the trypan blue staining assay for B16F10 cells, the cell viability by reticulol treatment was significantly decreased in a dose-dependent manner. The in vivo assay for the lung metastasis-blocking effect showed that reticulol injected intravenously suppressed the increase in colonies on the lung in a dose-dependent manner. In addition, the survival rate of tumor-implanted mice treated with reticulol was closely associated with its antitumoral efficacy. Reticulol administered via the peritoneum of mice showed less metastasis inhibition than that injected intravenously. To demonstrate the mechanism for inhibition of metastasis, the inhibitory effect of reticulol for matrix metalloproteinase-2 or -9 involved in melanoma metastasis was investigated; however, they were not observed on zymogram gel. In addition, the antitumor efficacy of reticulol was not associated with cell cycle arrest or apoptosis. Therefore, it was inferred that reticulol known as a phosphodiesterase inhibitor directly inhibited the growth of B16F10 melanoma, showing necrotic response. These results suggest that reticulol protects its lung metastasis via the bloodstream by inhibiting the growth of B16F10 melanoma at the cellular level.

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“…The phosphodiesterase inhibitor, reticulol [2,3], was found in the culture broth of a strain of Streptoverticillium sp. NA-4803 [1].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antitumor Efficacy of Reticulol from <i>Streptoverticillium</i> against the Lung Metastasis Model B16F10 Melanoma

et al. 2003

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“…The hydrolysis of the inhibitors was carried out with 6 N HCl at 110°C for 20 hours and the amino acid composition was determined with an amino acid analyzer. Amino acid analyses on their hydrolysates showed the same amino acid compositions to be glutamic acid (1), aspartic acid (1), valine (1) and leucine (4).…”

Section: Acylpeptides the Inhibitors Of Cyclic Adenosinementioning

confidence: 99%

“…4.17], inhibitors were isolated from the culture filtrate of Bacillus subtilis C-756. The isolated inhibitors were acylpeptides and had potent inhibitory activities compared with papaverine and theophylline, which are known as PDE inhibitors.…”

Section: Acylpeptides the Inhibitors Of Cyclic Adenosinementioning

confidence: 99%