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We evaluated the antiinflammatory activity of soy-derived di- and tripeptides in a dextran sodium sulfate (DSS)-induced pig model of intestinal inflammation. In the DSS-positive control (POS) and DSS-positive with soy peptide treatment (SOY) groups (n = 6/group), DSS was administered to piglets via i.g. catheter for 5 d, followed by a 5-d administration of saline or soy-derived peptides, respectively. A negative control (NEG) group received saline in lieu of the DSS and soy peptides. The severity of inflammation was assessed by clinical signs, morphological and histological measurements, gut permeability, and neutrophil infiltration. Local production of TNF and IL6 were measured by ELISA, colonic and ileal inflammatory gene expression were assessed by real-time RT-PCR, and CD4+CD25+ lymphocyte populations were analyzed by flow cytometry. Crypt elongation and muscle thickness, d-mannitol gut permeation, colonic expression of the inflammatory mediators IFNG, IL1B, TNF, RORC, and IL17A as well as the FOXP3 T-regulatory transcription factor, and myeloperoxidase activity were lower (P < 0.05) in the SOY pigs than in POS pigs. Messenger RNA levels of ileal IFNG, TNF, IL12B, and IL17A were lower (P < 0.05) and FOXP3 expression was greater (P < 0.05) in SOY piglets than in the POS group. In the mesenteric lymph nodes, CD4+CD25+ T cells were higher (P < 0.05) in both the POS and SOY groups than in NEG controls. Soy-derived peptides exert antiinflammatory activity in vivo, suggesting their usefulness for the treatment of inflammatory disorders.

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1. This study investigated the effects of beta1-4 Mannobiose (MNB)-supplemented feeds on the kinetics of Salmonella enterica serovar Enteritidis (SE) in broilers and the ensuing histopathological changes. D-Mannose (MAN) was used for comparison. The diets supplemented with MNB or MAN were fed during the first two weeks after hatching to investigate any protection against SE infection in growing birds and any immunomodulatory functions in the gut. 2. MNB-supplementation reduced SE organ colonisation, caecal carriage and faecal shedding in a time-dependent manner. The high concentrations and persistency of the SE-specific IgA response in those birds given rations supplemented with MNB or MAN were associated with a decline in SE shedding and caecal carriage in the later stages of infection. MNB was more effective against SE infection than MAN. 3. Histological examination of the caecal wall and caecal tonsils at 23 d post-infection indicated a lesser degree of intestinal pathology. An increased number of intra-epithelial mononuclear cells (mature lymphocytes and macrophages) in the lining epithelium of birds fed on the diet supplemented with MNB was accompanied by an increased number of lamina propria cells. 4. The present study indicates that feeding a diet supplemented with MNB during the first two weeks after hatching reduced susceptibility to SE infection. Supplementing the diet with MNB or MAN increased IgA production and improved SE clearance by acting as immunomodulatory agents that prevented intestinal pathology. Feeding a MNB-supplemented diet to broilers could be used as an alternative to antibiotics, because it has no adverse effects on mortality or weight gain.

PurposeThis study compared the effects of a combination of soy peptide supplementation and exercise with those of exercise only, on the cognitive function of elderly adults.Patients and methodsThis randomized, non-blinded, controlled clinical trial included 67 participants aged 60 years or more with non-cognitive dysfunction who were divided into two groups according to the intervention method: an exercise group (Ex group, n = 36) and an exercise plus nutrition group (Ex+Nt group, n = 31). The Ex group completed a memory training activity for 15 mins and aerobic exercise for 45 mins once a week for 90 days. The Ex+Nt group completed the same training plus received soy peptide for 90 days. The Mini-Mental Status Examination score, trail-making test A/B score, skeletal muscle mass index, grip strength, gait speed, and geriatric depression scale were measured at baseline and post intervention. For comparison between the pretest and posttest measurements to determine the intervention effects, a two-way analysis of variance was performed. The significance level was set at < 5%.ResultsA two-way analysis of variance revealed significant time effects on trail-making test-A score, skeletal muscle index, grip strength, and gait speed in both groups. There were significant time x group interactions for greater increase in calculation score.ConclusionA combination of exercise and soy peptide supplementation was effective in improving a portion of cognitive function.

β-1,4-Mannobiose (MNB) has been shown to exert prebiotic activity and modulate mucosal gene expression. In this study, the immune-modulating effect of MNB in healthy and endotoxemic mice and its role in Toll-like receptor (TLR) 2/4-mediated macrophage activation were investigated. Mice were supplemented daily with MNB (0, 5, 10, or 25 mg/kg) for 14 d. To examine the effect of MNB during endotoxemia, mice were supplemented with or without MNB (25 mg/kg) for 14 d, followed by challenge with intraperitoneal LPS or saline. MNB induced expression of both T helper (Th) 1- and Th2-type cytokines in the ileum (P < 0.05) and increased fecal IgA production and splenic NK cell activity (P < 0.05) in healthy mice. In endotoxemic mice, MNB reduced the expression of Tnfa, Il-6, iNos (P < 0.05), and Il-10 (P < 0.05), and reduced LPS-induced weight loss but increased Ifng, Il-12p40, Il-5, and Ifna expression (P < 0.05) and NK cell activity relative to positive control (LPS) mice. Treatment of RAW 264.7 macrophages with MNB induced TNF-α and IL-6 secretion (P < 0.05), and this effect was abrogated by inhibiting TLR4, but not TLR2, signaling. Pretreatment of RAW 264.7 cells with MNB induced tolerance to TLR2 and TLR4 agonists, reducing TNF-α production (P < 0.05) upon secondary stimulation with LPS or lipoteichoic acid. These results indicate that MNB can modulate intestinal and systemic immune responses in healthy and endotoxemic mice and prevent LPS-induced immune suppression, as well as directly stimulating innate immune mechanisms in vitro as a TLR4 agonist.

We evaluated the anti-inflammatory activity of mannanase-hydrolyzed copra meal (MNB), including β-1,4-mannobiose (67.8%), in a dextran sodium sulfate (DSS)-induced porcine model of intestinal inflammation. In the DSS-positive control (POS) and MNB treatment (MCM) groups, DSS was first administered to piglets via intragastric catheter for 5 days, followed by 5 days administration of saline or MCM. A negative control group (NEG) received a saline alternative to DSS and MNB. Inflammation was assessed by clinical signs, morphological and histological measurements, gut permeability and neutrophil infiltration. Local production of TNF-α and IL-6 were analyzed by ELISA, colonic and ileal inflammatory gene expressions were assessed by real time RT-PCR, and CD4+CD25+ cell populations were analyzed by flow cytometry. Crypt elongation and muscle thickness, D-mannitol gut permeation, colonic expression of the inflammatory mediators TNF-α and IL-6 and myeloperoxidase activity were significantly lower in the MCM group than in that of POS group. The mRNA levels of ileal IL-1β, IL-6, IL-17 and TNF-α were significantly lower following MCM treatment than with POS treatment.MNB exerts anti-inflammatory activity in vivo, suggesting that MNB is a novel therapeutic that may provide relief to human and animals suffering from intestinal inflammation.

We investigated the effects of dietary β-1,4-mannobiose (MNB) on the weights of body, breast muscles, thighs, liver, and abdominal adipose tissue in growing broiler chicks to evaluate whether MNB can be used as a feed additive for broiler chicks. A total of 24 eight day-old male broiler chicks were allocated to two cages and fed a control diet or a 0.01% MNB-supplemented diet for 14 days. Dietary MNB significantly increased the relative weight of breast muscles, whereas the weights of the body, thighs, liver and abdominal adipose tissue were not affected. The myostatin mRNA level in the breast muscle was significantly reduced by MNB. Since myostatin is a negative regulator of myoblast proliferation and differentiation, it is possible that the downregulation of myostatin gene expression is involved in the increased breast muscle growth with MNB. The plasma 3-methyl histidine level, which is known to be a nonmetabolizable amino acid marker of myofibrillar protein catabolism, and the breast muscle atrogin-1 mRNA level, which is involved in protein catabolism, were not affected by dietary MNB. In addition, MNB did not affect protease mRNA levels in the breast muscles. These results suggest that MNB does not affect proteolysis in the breast muscles. All our findings suggest that MNB is a promising candidate feed additive to improve the meat yield of broiler chickens.