Background: Recent observations in the EURODIAB Complications Study demonstrated that markers of insulin resistance are strong risk factors for retinopathy incidence in patients with diabetes. However, the molecular mechanism underlying this remains to be elucidated. In this study, we investigated the influence of palmitate, a major saturated free fatty acid in plasma, on the apoptotic cell death of cultured microvascular endothelial cells (EC) and retinal pericytes. Materials and Methods: The intracellular formation of reactive oxygen species (ROS) was detected using the fluorescent probe CM-H 2 DCFDA. DNA synthesis was determined by measuring [ 3 H]-thymidine incorporation into cells. DNA fragmentations of EC were quantitatively analyzed in an enzyme-linked immunosorbent

Alogliptin treatment improved the AGE-RAGE axis and reduced albuminuria in Japanese type 2 diabetes patients.

SUMMARYGenerally, a programmable LSI such as an FPGA is difficult to test compared to an ASIC. There are two major reasons for this. The first is that an automatic test pattern generator (ATPG) cannot be used because of the programmability of the FPGA. The other reason is that the FPGA architecture is very complex. In this paper, we propose a new FPGA architecture that will simplify the testing of the device. The base of our architecture is general island-style FPGA architecture, but it consists of a few types of circuit blocks and orderly wire connections. This paper also presents efficient test configurations for our proposed architecture. We evaluated our architecture and test configurations using a prototype chip. As a result, the chip was fully tested using our configurations in a short test time. Moreover, our architecture can provide comparable performance to a conventional FPGA architecture. key words: design for testability, homogeneous architecture, test method, prototype chip

We have studied the cellular localization of thyrotropin receptor (TSH-R) mRNA in orbital fat and extraocular muscle tissues from patients with thyroid-associated ophthalmopathy (TAO) using Northern blot, reverse transcriptase polymerase chain reaction (RT-PCR), and in situ hybridization, and we correlated the findings with clinical estimates of ophthalmopathy. Although we failed to detect TSH-R mRNA in orbital tissues by Northern blot, TSH-R cDNA was amplified in orbital fat tissue from 13 of 25 patients with TAO and from 2 of 4 control subjects, in eye muscle tissue from 2 out of 7 patients with TAO, and in cultured orbital fibroblasts and subcutaneous fibroblasts from TAO patients. In situ hybridization showed that TSH-R mRNA was detected in cultured orbital fibroblasts as well as skin fibroblasts obtained from the patient. Furthermore, the expression of TSH-R mRNA in orbital fat tissue from patients with TAO significantly correlated with the orbital fat volume and the severity of ophthalmopathy, especially the extent of eye muscle dysfunction. These results suggest that the expression of TSH-R in the orbit, especially fibroblasts, may play a role in the pathogenesis and clinical manifestations of the ophthalmopathy in patients with TAO, although a secondary effect, involving fibroblasts in TAO is also possible.

Among older hypertensive patients with antihypertensive medications, those who had echocardiographically determined cardiac hypertrophy may be at high risk for cognitive dysfunction, irrespective of their office BP and 24-h BP levels.