The anticorrosion effect and biocompatibility property of Poly (methyl methacrylate) (PMMA) materials have been used in a variety of commercially available products and medical devices for many years. In this work, the treatment of surfaces prepared PMMA films by oxygen, argon and nitrogen plasma, requires understanding the effect of low energy ions on the surface modification of the film of PMMA. Due to this, the samples of prepared PMMA were exposed to three different gases at the same discharge conditions. Changes within the morphology and surface hydrophilicity of the treated samples were characterized by optical microscope images and the measurements of contact angle. Treatment of PMMA with O2, N2, Ar gases displayed a decreasing in the contact angle that results in increasing surface free energy. Oxygen –plasma treatment of PMMA films leads to high aspect ratio topography, with increased roughness for (5-30) minute process durations.
The diabetes can be induced by injecting “Alloxan” into animals. it shows a good response in animals specially in “mice, rabbits, mice, dog, and rats”. The drug dosage is between “90 and 200mg/kg of the body weight”. The “Alloxan” motivate “blood glucose” and the experimental animals shows response to the injected drug. The samples which are used in this study are mice male gender with age ranging between three and four months and weight (20-25)gm, these mice are injected with “Alloxan” with a dose of alloxan(200mg/kg) to study effect of alloxan on blood compounds (The cells of white blood, the cells of Red blood and platelets) and study the effect of plasma jet on the infected mice for different time exposure, the component of blood shows different response to the plasma jet effect the White Blood Cells (WBC) and the Platelets(PLT) approach the normal case in the 30 second, the Red Blood Cells (RBC) , HGB the concentration of hemoglobin in whole blood and (HCT) the ratio of red blood cells to the volume of blood plasma approach the normal case in 50 second, the (MCV) mean cell volume approach the normal value in 10 second.
Non-thermal plasma(Dielectric barrier discharge) has many uses including living tissue sterilization, inactivation of the bacteria, excimer formation, angiogenesis, and surface treatment. This research aim is to use cold plasma as a tool to search the effect of the dielectric barrier discharge system at room temperature on human sperm motility and DNA integrity. This work was performed on 60 human semen samples suffering from low motility; each sample was prepared by centrifugation method, then each semen sample was divided into two sections, the first section is before significant exposure to the plasma system (DBD) and the second section is after treatment with the DBD system at ambient temperature. Before and after exposure to non-thermal Plasma (DBD), DNA integrity and sperm motility were assessed, patients suffered from asthenospermia has a high level of DNA fragmentation than fertile male, ( 24.16±4.14) p<0.001 for untreated and after treatment the semen slide with dielectric barrier discharge the percentage decreases to (9.16±1.76) p>0.006, and the comet slide was (35.44±4.15) then the percentage decline to (19.86 ±2.44) these results have shown that cold plasma improves sperm motility and decreases from DNA damage in patients with medium and high level of DNA damage.
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