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Mainstream anaerobic treatment of domestic wastewater is a promising energy-generating treatment strategy; however, such reactors operated in colder regions are not well characterized. Performance data from a pilot-scale, multiple-compartment anaerobic reactor taken over 786 days were subjected to comprehensive statistical analyses. Results suggest that chemical oxygen demand (COD) was a poor proxy for organics in anaerobic systems as oxygen demand from dissolved inorganic material, dissolved methane, and colloidal material influence dissolved and particulate COD measurements. Additionally, univariate and functional boxplots were useful in visualizing variability in contaminant concentrations and identifying statistical outliers. Further, significantly different dissolved organic removal and methane production was observed between operational years, suggesting that anaerobic reactor systems may not achieve steady-state performance within one year. Last, modeling multiple-compartment reactor systems will require data collected over at least two years to capture seasonal variations of the major anaerobic microbial functions occurring within each reactor compartment.

Modeled electrical energy generation from anaerobic primary treatment of domestic wastewater with baffled bioreactors. Energy generation from methane production exceeded energy use by conventional activated sludge in some scenarios.

In this study, the abundance and sequences of the amoA gene in ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA) were defined in three wastewater treatment plants using activated sludge with biological nitrogen removal in different countries: Thailand, United States of America (USA), and Japan. Quantitative real-time polymerase chain reaction (PCR) and PCR coupled with denaturing gradient gel electrophoresis were used to find the comparative abundance and identity of AOB and AOA. The conditions at the Phuket WWTP in Thailand promoted the dominance of AOA amoA genes over AOB amoA genes, while conditions at the WWTPs in Japan and USA promoted growth of AOB. Three parameters that may have contributed to the AOA dominance in Phuket were longer SRT, higher temperature, and higher pH. The Phuket WWTP is a unique system that can be used to better understand the conditions that promote AOA growth and dominance over AOB. In addition, analysis of operational data in conjunction with AOA and AOB community structure from the Phuket WWTP may elucidate advantages of AOA in meeting stricter treatment standards.

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Two full scale systems of oxidation ditches for domestic wastewater treatment plants (WWTP) were used as study sites: Phuket Province, southern Thailand (representative of tropical humid climates) and Plum Creek, Castle Rock, Colorado, USA (representative of cold climates). The treatment systems at both sites were designed for biological nutrient removal (BNR) fromextended activated sludge. Nitrogen is removed by nitrification-denitrification processes. The solid retention time (SRT) for both treatment plants was ≥ 10 das recommended by theory for complete nitrification in activated sludge wastewater treatment plants. Influents and effluents from these sites were compared in respect to flow rate, biochemical oxygen demand (BOD), organic nitrogen, ammonium, nitrate, total nitrogen, and phosphorus concentrations. At both sites, nutrient removal reached more than 75% because there was sufficient carbon for denitrifying and phosphate accumulating organisms. Furthermore, low dissolved oxygen concentration, long SRT, and hightemperature could be key factors to promote activity of some groups of bacteria in consuming organic matter and nutrients in wastewater in warm climates. For this reason, plant design and operating procedures for wastewater treatment in cold climates might not be always be applicable to warm climates.