Background:
- According to the World Health Organization (WHO), two
fifths of the world population is at risk of infection by DENV. There are
not any safe and effective vaccine established. Sulfated
glycosaminoglycans such as heparin, used as anticoagulant inhibit the
initial step of dengue viral replication. Recently, an isolated heparin
analogue Goniopsis cruentata (cCTH) has presented low anticoagulant
effect with reduced bleeding risk.
Methods :
- The antiviral activity of cCTH and heparin compounds against
DENV-2 in Vero cell culture was determined by quantitative RT-PCR
(qRT-PCR) and titration. For this, it was carried out four trials: treatment of
the cells for 2 h before viral inoculation, concomitant viral inoculation
treatment, treatment after viral inoculation and virucidal assay.
Subsequently, the culture supernatants were collected for periods of 24, 48
and 72 h.
Results:
- Our results have been demonstrated that cCTH and heparin
showed antiviral activity against DENV-2.
Conclusion :
- These data suggest that both compounds prevented viral
replication in cultured Vero cells.
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