A simple, accurate, and reproducible high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20 A HPLC system equipped with a Supelcosil LC-18 T C column and an isocratic mobile phase consisting of 0.3% trichloroacetic acid in water and acetonitrile HPLC-grade (50:50, v/v) run at a flow rate of 0.9 mL/min for 13 min. The UV detection wavelength was set at 254 nm. The method exhibited good linearity (R > 0.994) over the assayed concentration range (0.10-25 μg/mL) and demonstrated good intra-day and inter-day precision and accuracy (relative standard deviations and the deviation from predicted values were <20%). This method was also successfully applied for studying the pharmacokinetics of QR in rats following a single oral dose of QR to evaluate its pharmacokinetic parameters in rats.
The paper represents the results of the study of the morphological features and the spread of Viscum coloratum (Kom.) Nakai in East Asia in order to identify the specific features of this species and to establish the northern and northwestern boundaries of its range. Ornamental mistletoe in the Far East is represented by two forms: forma lutescens Kitag. and the rubroaurantiacum (Makino) Kitag. As a result of our study, it was established that the north-western boundary of the distribution of ornamental mistletoe reaches the valley of the middle course of the Zeya River in the Amur Region. It should be noted for the first time that Viscum coloratum grows on Quercus mongolica (Mongolian oak). Mistletoe abundantly affects the linden trees near the forest apiaries and in the period of 20 years almost inhabits most of the trees and forces the beekeepers to transfers apiaries to other places. Among the distributors of the fruits of Viscum coloratum, ordinary and Japanese waxwings predominate. In folk medicine of East Asian countries mistletoe is known to be used in the treatment of hypertension and oncological diseases. The main antitumor substances in the water extract of mistletoe are lectins.
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