A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats

Abdelkawy, Khaled; Balyshev, Mark E.; Elbarbry, Fawzy

doi:10.1002/bmc.3819

Cited by 39 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, fresh QR solutions were made in 5% Pluronic ® F127 every three days. Further, our previous studies have indicated that the oral bioavailability of QR in a SHR rat model is comparable to reported bioavailability data in humans [ 21 ]. The selection of the QR concentration in drinking water was designed to mimic the average daily servings of quercetin, i.e., clinically relevant doses, taking into consideration the average daily water intake and predicted oral bioavailability of QR.…”

Section: Discussionsupporting

confidence: 76%

The Antihypertensive Effect of Quercetin in Young Spontaneously Hypertensive Rats; Role of Arachidonic Acid Metabolism

Elbarbry

Abdelkawy

Moshirian

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Hypertension affects almost 50% of the adult American population. Metabolites of arachidonic acid (AA) in the kidney play an important role in blood pressure regulation. The present study investigates the blood pressure-lowering potential of quercetin (QR), a naturally occurring polyphenol, and examines its correlation to the modulation of AA metabolism. Spontaneously hypertensive rats (SHR) were randomly divided into four groups. Treatment groups were administered QR in drinking water at concentrations of 10, 30, and 60 mg/L. Blood pressure was monitored at seven-day intervals. After a total of seven weeks of treatment, rats were killed and kidney tissues were collected to examine the activity of the two major enzymes involved in AA metabolism in the kidney, namely cytochrome P450 (CYP)4A and soluble epoxide hydrolase (sEH). Medium- and high-dose QR resisted the rise in blood pressure observed in the untreated SHR and significantly inhibited the activity of the CYP4A enzyme in renal cortical microsomes. The activity of the sEH enzyme in renal cortical cytosols was significantly inhibited only by the high QR dose. Our data not only demonstrate the antihypertensive effect of QR, but also provide a novel mechanism for its underlying cardioprotective properties.

show abstract

Section: Discussionsupporting

confidence: 76%

The Antihypertensive Effect of Quercetin in Young Spontaneously Hypertensive Rats; Role of Arachidonic Acid Metabolism

Elbarbry

Abdelkawy

Moshirian

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…We employed different flavonoid/GSH ratios—from equal to a great excess of GSH. Although the total dietary flavonoid intake can be greater than GSH, the expected concentration of individual flavonoids in an organism is always much less than GSH [ 8 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Flavonoids with Glutathione Antioxidant Synergy: Influence of Free Radicals Inflow

et al. 2020

View full text Add to dashboard Cite

This report explores the antioxidant interaction of combinations of flavonoid–glutathione with different ratios. Two different 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical (ABTS•+)-based approaches were applied for the elucidation of the antioxidant capacity of the combinations. Despite using the same radical, the two approaches employ different free radical inflow systems: An instant, great excess of radicals in the end-point decolorization assay, and a steady inflow of radicals in the lag-time assay. As expected, the flavonoid–glutathione pairs showed contrasting results in these two approaches. All the examined combinations showed additive or light subadditive antioxidant capacity effects in the decolorization assay. This effect showed slight dilution dependence and did not change when the initial ABTS•+ concentration was two times as high or low. However, in the lag-time assay, different types of interaction were detected, from subadditivity to considerable synergy. Taxifolin–glutathione combinations demonstrated the greatest synergy, at up to 112%; quercetin and rutin, in combination with glutathione, revealed moderate synergy in the 30–70% range; while morin–glutathione appeared to be additive or subadditive. In general, this study demonstrated that, on the one hand, the effect of flavonoid–glutathione combinations depends both on the flavonoid structure and molar ratio; on the other hand, the manifestation of the synergy of the combination strongly depends on the mode of inflow of the free radicals.

show abstract

“…Quercetin (3,3′,4′,5,7-pentahydroxyflavone), a major bioflavonoid found in fruits and vegetables, exhibits unique biological properties [ 1 ]. A broad spectrum of beneficial quercetin properties, including antimutagenic, antifibrotic, antiinflammatory, antiatherogenic, and antibacterial effects, as well as its strong antioxidative capacity, is described.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity In Vitro of Quercetin Glycoside Obtained in Beauveria bassiana Culture and Its Interaction with Liposome Membranes

et al. 2017

View full text Add to dashboard Cite

Quercetin (Q) was used as substrate for regioselective glycosylation at the C-7 position catalyzed by Beauveria bassiana AM278 strain. As a result the glycoside quercetin 7-O-β-d-(4″-O-methyl)glucopyranoside (Q 7-MeGlu) was formed. The goal of the studies was to determine the anti-oxidative (liposome membrane protection against free radicals IC50Q 7-MeGlu = 5.47 and IC50Q = 4.49 µM) and anti-inflammatory (COX-1 and COX-2 enzymes activity inhibition) properties of Q 7-MeGlu as compared to Q. Every attempt was made to clarify the antioxidant activity of these molecules, which are able to interact with egg phosphatidylcholine liposomes, using a fluorometric method (by applying the probes MC540, TMA-DPH and DPH). The results indicated that Q 7-MeGlu and Q are responsible for increasing the packing order, mainly in the hydrophilic but also in hydrophobic regions of the membrane (Q > Q 7-MeGlu). These observations, confirmed by a 1H-NMR method, are key to understanding their antioxidant activity which is probably caused by the stabilizing effect on the lipid membranes. The results showed that Q 7-MeGlu and Q have ability to quench the human serum albumin (HSA) intrinsic fluorescence through a static quenching mechanism. The results of thermodynamic parameters indicated that the process of formation complexes between studied molecules and HSA was spontaneous and caused through Van der Waals interactions and hydrogen bonding.

show abstract

A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats

Cited by 39 publications

References 24 publications

The Antihypertensive Effect of Quercetin in Young Spontaneously Hypertensive Rats; Role of Arachidonic Acid Metabolism

The Antihypertensive Effect of Quercetin in Young Spontaneously Hypertensive Rats; Role of Arachidonic Acid Metabolism

Flavonoids with Glutathione Antioxidant Synergy: Influence of Free Radicals Inflow

Bioactivity In Vitro of Quercetin Glycoside Obtained in Beauveria bassiana Culture and Its Interaction with Liposome Membranes

Contact Info

Product

Resources

About